Document Detail

Cryopreservation of primary cell cultures of marine invertebrates.
MedLine Citation:
PMID:  11788872     Owner:  NLM     Status:  MEDLINE    
Primary cell cultures obtained from somatic and larval tissues of bivalve molluscs and from embryos of sea urchins were frozen to -196 degrees C by two-step freezing using 10% dimethyl sulfoxide (DMSO) or/and trehalose (3-30 mg/ml) as cryoprotectants. We estimated both cell viability and the RNA synthetic activity after freeze-thaw. Total lipid extracts from the tissues of echinoderms examined as possible cryoprotective agents demonstrated a weak cryoprotective capacity. Mussel lipid extract was found to possess a considerable cryoprotective activity. Cryoprotective capacity of tested lipids correlated with their thermotropic behaviour. DMSO + trehalose combination was shown to be a favourable cryoprotectant and sea urchin blastula cells the most freezing-tolerant cells.
N Odintsova; K Kiselev; N Sanina; E Kostetsky
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cryo letters     Volume:  22     ISSN:  0143-2044     ISO Abbreviation:  Cryo Letters     Publication Date:    2001 Sep-Oct
Date Detail:
Created Date:  2002-01-14     Completed Date:  2002-01-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9891832     Medline TA:  Cryo Letters     Country:  England    
Other Details:
Languages:  eng     Pagination:  299-310     Citation Subset:  IM    
Institute of Marine Biology, Far East Branch of Russian Academy of Sciences, Vladivostok, Russia 690041.
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MeSH Terms
Cell Survival
Cells, Cultured
Cryoprotective Agents
Dimethyl Sulfoxide
Embryo, Nonmammalian
Gills / cytology,  metabolism
Mollusca / cytology*
Muscles / cytology,  metabolism
RNA / biosynthesis
Sea Urchins / cytology*
Uridine / metabolism
Reg. No./Substance:
0/Cryoprotective Agents; 58-96-8/Uridine; 63231-63-0/RNA; 67-68-5/Dimethyl Sulfoxide; 99-20-7/Trehalose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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