Document Detail

Cryopreservation of the non-dormant orthodox seeds of Ulmus glabra.
MedLine Citation:
PMID:  20519176     Owner:  NLM     Status:  MEDLINE    
In order to evaluate the feasibility of cryopreservation of Wych elm ( Ulmus glabra Huds.) seeds, we evaluated the seeds sensitivity to extreme desiccation and/or the ultra-low temperature of liquid nitrogen (LN; -196 degrees C). We also determined the critical water content (WC) of desiccated seeds and the high-moisture freezing limit of seeds desiccated or moistened to various WCs and frozen for 24 h or up to two years in LN. Germination tests revealed no critical WC for seeds to 0.03 g H 2 O g -1 dry mass, g g -1 . Seeds tolerated freezing in LN within safe ranges of WC 0.03-0.21 g g -1 (nuts). Seeds desiccated to the safe WC and stored in LN for two years had similar germination as seeds stored at -3 degrees C for two years. Therefore, long-term cryopreservation of U. glabra seeds in gene banks is feasible.
P Chmielarz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta biologica Hungarica     Volume:  61     ISSN:  0236-5383     ISO Abbreviation:  Acta. Biol. Hung.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-06-03     Completed Date:  2010-08-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8404358     Medline TA:  Acta Biol Hung     Country:  Hungary    
Other Details:
Languages:  eng     Pagination:  224-33     Citation Subset:  IM    
Polish Academy of Sciences Institute of Dendrology Parkowa 5 62-035 Kórnik Poland.
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MeSH Terms
Cryoprotective Agents / pharmacology*
Nitrogen / chemistry
Seeds / chemistry,  metabolism*
Time Factors
Ulmus / metabolism*
Water / chemistry
Reg. No./Substance:
0/Cryoprotective Agents; 7727-37-9/Nitrogen; 7732-18-5/Water

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