Document Detail


Cryopreservation of the non-dormant orthodox seeds of Ulmus glabra.
MedLine Citation:
PMID:  20519176     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to evaluate the feasibility of cryopreservation of Wych elm ( Ulmus glabra Huds.) seeds, we evaluated the seeds sensitivity to extreme desiccation and/or the ultra-low temperature of liquid nitrogen (LN; -196 degrees C). We also determined the critical water content (WC) of desiccated seeds and the high-moisture freezing limit of seeds desiccated or moistened to various WCs and frozen for 24 h or up to two years in LN. Germination tests revealed no critical WC for seeds to 0.03 g H 2 O g -1 dry mass, g g -1 . Seeds tolerated freezing in LN within safe ranges of WC 0.03-0.21 g g -1 (nuts). Seeds desiccated to the safe WC and stored in LN for two years had similar germination as seeds stored at -3 degrees C for two years. Therefore, long-term cryopreservation of U. glabra seeds in gene banks is feasible.
Authors:
P Chmielarz
Related Documents :
25302866 - Electrochemical behavior of platinum nanoparticles on a carbon xerogel support modified...
9642876 - In vitro viability of mouse oocytes vitrified in an ethylene glycol-based solution.
2374136 - A simple method for mouse embryo cryopreservation in a low toxicity vitrification solut...
12739776 - Effective inhibition of mannitol crystallization in frozen solutions by sodium chloride.
21267706 - Surface-induced breakout crystallization in cylinder-forming p(i-b-eo) diblock copolyme...
16509656 - Product screening of fast reactions in ir-laser-heated liquid water filaments in a vacu...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta biologica Hungarica     Volume:  61     ISSN:  0236-5383     ISO Abbreviation:  Acta. Biol. Hung.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-06-03     Completed Date:  2010-08-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8404358     Medline TA:  Acta Biol Hung     Country:  Hungary    
Other Details:
Languages:  eng     Pagination:  224-33     Citation Subset:  IM    
Affiliation:
Polish Academy of Sciences Institute of Dendrology Parkowa 5 62-035 Kórnik Poland. pach@man.poznan.pl
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Cryopreservation
Cryoprotective Agents / pharmacology*
Desiccation
Germination
Nitrogen / chemistry
Seeds / chemistry,  metabolism*
Temperature
Time Factors
Ulmus / metabolism*
Water / chemistry
Chemical
Reg. No./Substance:
0/Cryoprotective Agents; 7727-37-9/Nitrogen; 7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Towards reducing genotype specificity in regeneration protocols after somatic hybridization between ...
Next Document:  Changes in carbohydrate content of potato calli during osmotic stress induced by mannitol.