Document Detail

Cryopreservation of mouse spermatozoa in the presence of raffinose and glycerol.
MedLine Citation:
PMID:  2401978     Owner:  NLM     Status:  MEDLINE    
When mouse epididymal spermatozoa were rapidly frozen in two steps (37 to -70 degrees C for solid CO2 and -70 to -196 degrees C for liquid nitrogen) as pellets, 18% raffinose provided the greatest protection to ICR mouse spermatozoa against cold-shock; sperm motility and fertilizing ability were 43% and 22.4%, respectively. A small proportion of spermatozoa frozen with 10% sucrose was motile but incapable of fertilizing ovulated oocytes. Glycerol and dimethylsulphoxide were less effective at any concentration examined. However, the fertilizing ability of frozen-thawed ICR spermatozoa was significantly improved (35.5%) by addition of glycerol (1.75% final concentration) to medium containing 18% raffinose. Spermatozoa from one outbred (ddY) and 5 inbred (C57BL/6N, C3H/HeN, DBA/2N, BALB/c and kk) strains of mice were successfully frozen in the presence of 18% raffinose and 1.75% glycerol, although the fertilization rates of frozen-thawed spermatozoa varied among strains (13% for C57BL/6N to 64% for DBA/2N). A small fraction of mouse eggs resulting from fertilization by frozen-thawed spermatozoa developed normally in vitro (37% in C57BL/6N to 71% in ICR) to the blastocyst stage and in vivo (19% for C57BL/6N spermatozoa and ddY oocytes) to Day 18 of gestation.
N Tada; M Sato; J Yamanoi; T Mizorogi; K Kasai; S Ogawa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of reproduction and fertility     Volume:  89     ISSN:  0022-4251     ISO Abbreviation:  J. Reprod. Fertil.     Publication Date:  1990 Jul 
Date Detail:
Created Date:  1990-10-24     Completed Date:  1990-10-24     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0376367     Medline TA:  J Reprod Fertil     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  511-6     Citation Subset:  IM    
Section of Experimental Animal Administration, Hoechst Japan Limited, Saitama.
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MeSH Terms
Blastocyst / physiology
Cell Survival
Cryopreservation / methods*
Cryoprotective Agents / pharmacology*
Fertilization in Vitro
Glycerol / pharmacology
Mice, Inbred ICR
Oligosaccharides / pharmacology*
Raffinose / pharmacology*
Reg. No./Substance:
0/Cryoprotective Agents; 0/Oligosaccharides; 512-69-6/Raffinose; 56-81-5/Glycerol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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