| Cryopreservation of mouse spermatozoa in the presence of raffinose and glycerol. | |
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MedLine Citation:
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PMID: 2401978 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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When mouse epididymal spermatozoa were rapidly frozen in two steps (37 to -70 degrees C for solid CO2 and -70 to -196 degrees C for liquid nitrogen) as pellets, 18% raffinose provided the greatest protection to ICR mouse spermatozoa against cold-shock; sperm motility and fertilizing ability were 43% and 22.4%, respectively. A small proportion of spermatozoa frozen with 10% sucrose was motile but incapable of fertilizing ovulated oocytes. Glycerol and dimethylsulphoxide were less effective at any concentration examined. However, the fertilizing ability of frozen-thawed ICR spermatozoa was significantly improved (35.5%) by addition of glycerol (1.75% final concentration) to medium containing 18% raffinose. Spermatozoa from one outbred (ddY) and 5 inbred (C57BL/6N, C3H/HeN, DBA/2N, BALB/c and kk) strains of mice were successfully frozen in the presence of 18% raffinose and 1.75% glycerol, although the fertilization rates of frozen-thawed spermatozoa varied among strains (13% for C57BL/6N to 64% for DBA/2N). A small fraction of mouse eggs resulting from fertilization by frozen-thawed spermatozoa developed normally in vitro (37% in C57BL/6N to 71% in ICR) to the blastocyst stage and in vivo (19% for C57BL/6N spermatozoa and ddY oocytes) to Day 18 of gestation. |
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Authors:
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N Tada; M Sato; J Yamanoi; T Mizorogi; K Kasai; S Ogawa |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of reproduction and fertility Volume: 89 ISSN: 0022-4251 ISO Abbreviation: J. Reprod. Fertil. Publication Date: 1990 Jul |
Date Detail:
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Created Date: 1990-10-24 Completed Date: 1990-10-24 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0376367 Medline TA: J Reprod Fertil Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 511-6 Citation Subset: IM |
Affiliation:
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Section of Experimental Animal Administration, Hoechst Japan Limited, Saitama. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blastocyst / physiology Cell Survival Cryopreservation / methods* Cryoprotective Agents / pharmacology* Fertilization in Vitro Glycerol / pharmacology Male Mice Mice, Inbred ICR Oligosaccharides / pharmacology* Raffinose / pharmacology* Spermatozoa* |
| Chemical | |
Reg. No./Substance:
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0/Cryoprotective Agents; 0/Oligosaccharides; 512-69-6/Raffinose; 56-81-5/Glycerol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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