| Cryopreservation methodologies: freezing location and protocol alter development of mouse embryos. | |
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MedLine Citation:
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PMID: 14984286 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The purpose of this study was to determine the effects of location in the freezing chamber and various freezing protocols on post-thaw development of two-cell mouse embryos. Different protocols were used to cryopreserve two-cell mouse embryos in different freezer positions. Embryos were thawed and cultured to observe post-thaw development. When four different freezer positions were compared, one position provided better post-thaw blastocyst development, regardless of several protocols tested. Furthermore, there were significant differences in temperatures between positions in the freezer. These differences came before seeding (-7 degrees C), just before changing the -7.0 degrees C hold demand (Ramp 2), during the two time points tested during Ramp 3 (-0.3 degrees C/min), and during the holding ramp of -30 degrees C. Post-thaw embryo development was improved by lengthening the time after seeding but before decreasing the temperature again. Decreasing embryos' temperature before plunging appeared to improve post-thaw performance. |
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Authors:
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William R Boone; Jane E Johnson; Dawn W Blackhurst; H Lee Higdon III |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Contemporary topics in laboratory animal science / American Association for Laboratory Animal Science Volume: 43 ISSN: 1060-0558 ISO Abbreviation: Contemp Top Lab Anim Sci Publication Date: 2004 Jan |
Date Detail:
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Created Date: 2004-02-26 Completed Date: 2004-03-16 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9204153 Medline TA: Contemp Top Lab Anim Sci Country: United States |
Other Details:
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Languages: eng Pagination: 26-31 Citation Subset: IM |
Affiliation:
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Reproductive Endocrinology and Infertility, Greenville Hospital System, 890 W. Faris Rd., Suite 470, Greenville, South Carolina 29605-5601, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cold Temperature Cryopreservation / instrumentation, methods*, veterinary* Embryonic and Fetal Development / physiology* Mice Time Factors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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