Document Detail


Cryopreservation methodologies: freezing location and protocol alter development of mouse embryos.
MedLine Citation:
PMID:  14984286     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The purpose of this study was to determine the effects of location in the freezing chamber and various freezing protocols on post-thaw development of two-cell mouse embryos. Different protocols were used to cryopreserve two-cell mouse embryos in different freezer positions. Embryos were thawed and cultured to observe post-thaw development. When four different freezer positions were compared, one position provided better post-thaw blastocyst development, regardless of several protocols tested. Furthermore, there were significant differences in temperatures between positions in the freezer. These differences came before seeding (-7 degrees C), just before changing the -7.0 degrees C hold demand (Ramp 2), during the two time points tested during Ramp 3 (-0.3 degrees C/min), and during the holding ramp of -30 degrees C. Post-thaw embryo development was improved by lengthening the time after seeding but before decreasing the temperature again. Decreasing embryos' temperature before plunging appeared to improve post-thaw performance.
Authors:
William R Boone; Jane E Johnson; Dawn W Blackhurst; H Lee Higdon III
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Contemporary topics in laboratory animal science / American Association for Laboratory Animal Science     Volume:  43     ISSN:  1060-0558     ISO Abbreviation:  Contemp Top Lab Anim Sci     Publication Date:  2004 Jan 
Date Detail:
Created Date:  2004-02-26     Completed Date:  2004-03-16     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9204153     Medline TA:  Contemp Top Lab Anim Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  26-31     Citation Subset:  IM    
Affiliation:
Reproductive Endocrinology and Infertility, Greenville Hospital System, 890 W. Faris Rd., Suite 470, Greenville, South Carolina 29605-5601, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cold Temperature
Cryopreservation / instrumentation,  methods*,  veterinary*
Embryonic and Fetal Development / physiology*
Mice
Time Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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