Document Detail


Cryopreservation of the mammalian kidney. II. Demonstration of immediate ex vivo function after introduction and removal of 7.5 M cryoprotectant.
MedLine Citation:
PMID:  9299103     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The objective of the present study was to determine whether rabbit kidneys could be perfused with a 7.5 M vitrification solution (VS4, which vitrifies under applied pressure) without loss of function. To answer this question, kidneys were perfused with VS4 using a computer-based machine to gradually raise and lower concentration and then attached to the aorta and vena cava of a perfusor rabbit using an apparatus that permitted renal blood flow and renal function to be measured. About half (6/13) of the kidneys so evaluated resumed substantial immediate function after a transient period of severely reduced blood flow. Loss of function did not occur if cryoprotectant concentration was limited to 3.8 M. The loss of function produced by VS4 could be partially reproduced by artificially limiting blood reflow in control kidneys to simulate the transiently low flows caused by VS4 exposure. These results provide the first evidence that both the parenchyma and the vascular system of a sensitive mammalian organ can survive exposure to a vitrifiable concentration of cryoprotectant.
Authors:
G M Fahy; S E Ali
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cryobiology     Volume:  35     ISSN:  0011-2240     ISO Abbreviation:  Cryobiology     Publication Date:  1997 Sep 
Date Detail:
Created Date:  1997-10-23     Completed Date:  1997-10-23     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  114-31     Citation Subset:  IM    
Copyright Information:
Copyright 1997 Academic Press.
Affiliation:
Transfusion and Cryopreservation Research Program, Naval Medical Research Institute, Building 29, 8901 Wisconsin Avenue, Bethesda, Maryland 20889, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cryopreservation / methods*
Cryoprotective Agents
Female
Glomerular Filtration Rate
Kidney* / anatomy & histology,  blood supply,  physiology
Kidney Transplantation
Male
Organ Preservation / methods*
Perfusion
Rabbits
Renal Circulation
Renin / secretion
Solutions
Vascular Resistance
Grant Support
ID/Acronym/Agency:
BSRG 2 S07 RR05737/RR/NCRR NIH HHS; GM 17959/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Cryoprotective Agents; 0/Solutions; EC 3.4.23.15/Renin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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