| Cryopreservation of mammalian embryos: Advancement of putting life on hold. | |
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MedLine Citation:
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PMID: 20860056 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rodent transgenesis and human-assisted reproductive programs involve multistep handling of preimplantation embryos. The efficacy of production and quality of results from conventionally scheduled programs are limited by temporal constraints other than the quality and quantities of embryos per se. The emergence of vitrification, a water ice-free cryopreservation technique, as a reliable way to arrest further growth of preimplantation embryos, provides an option to eliminate the time constraint. In this article, current and potential applications of cryopreservation to facilitate laboratory animal experiments, colony management, and human-assisted reproductive programs are reviewed. Carrier devices developed for vitrification in the last two decades are compared with an emphasis on their physical properties that infer cooling rate of samples and sterility assurance. Biological impacts of improved cryopreservation on preimplantation embryos are also discussed. |
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Authors:
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Wai Hung Tsang; King L Chow |
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Publication Detail:
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Type: Journal Article; Review |
Journal Detail:
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Title: Birth defects research. Part C, Embryo today : reviews Volume: 90 ISSN: 1542-9768 ISO Abbreviation: Birth Defects Res. C Embryo Today Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-09-22 Completed Date: 2010-12-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101167665 Medline TA: Birth Defects Res C Embryo Today Country: United States |
Other Details:
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Languages: eng Pagination: 163-75 Citation Subset: IM |
Copyright Information:
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© 2010 Wiley-Liss, Inc. |
Affiliation:
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Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blastocyst / metabolism* Cryopreservation* Embryo, Mammalian / embryology* Female Gene Transfer Techniques* Humans Mice Models, Animal Reproductive Techniques, Assisted Vitrification* |
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