Document Detail


Cryopreservation of lymphocytes for use in in vitro assays of cellular immunity.
MedLine Citation:
PMID:  130427     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human lymphocytes can be cryopreserved for the purpose of performing in vitro tests of cellular immunity. A systematic study of the conditions for freezing and recovering cells has shown that there is wide flexibility in cell concentrations of cryoprotective agent and the temperature and rate of dilution however, have definable optima. Cells frozen and thawed under optimal conditions retain their reactivity in MLC and can be used for sequential studies of immune responsiveness. Approximately 70% of viable and functional cells are recovered when the cells are frozen in 7.5--12.5% DMSO and no further cryoprotection is discernable when up to 50% serum is added to the freezing media. The temperature and rate of dilution are critical only in that cells diluted rapidly in THE COLD (10-fold in 2 min at 0 degrees C) are less responsive in MLC than are cells diluted slowly (10-fold in 10 min) in the cold or slowly or rapidly at room temperature.
Authors:
R S Weiner
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of immunological methods     Volume:  10     ISSN:  0022-1759     ISO Abbreviation:  J. Immunol. Methods     Publication Date:  1976  
Date Detail:
Created Date:  1976-05-18     Completed Date:  1976-05-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1305440     Medline TA:  J Immunol Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  49-60     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Survival
Freezing
Humans
Lymphocyte Activation
Lymphocyte Culture Test, Mixed
Lymphocytes / immunology*
Temperature

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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