Document Detail

Cryopreservation of living cells: principles and practice.
MedLine Citation:
PMID:  17465961     Owner:  NLM     Status:  MEDLINE    
Increasingly, the cryopreservation of living cells is being attempted by researchers whose primary interest and experience is with the medical applications of those cells or tissues and whose prior experience with cryobiology may be negligible. It is therefore generally necessary to imitate some regimen used by others, perhaps with some other cell type and attempt to optimize the recovery empirically. This article makes no attempt to provide specific protocols for the many individual cell types. Rather it is a primer that may help to give such investigators an insight into the basic principles of cell freezing, cryoprotectants, and, particularly, their addition and removal. Finally, the article summarizes the five different approaches to applied cryopreservation: ultrarapid freezing and thawing, controlled-rate freezing, freezing with nonpenetrating polymers, vitrification, and equilibrium freezing.
Harold T Meryman
Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Transfusion     Volume:  47     ISSN:  0041-1132     ISO Abbreviation:  Transfusion     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-04-30     Completed Date:  2007-09-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0417360     Medline TA:  Transfusion     Country:  United States    
Other Details:
Languages:  eng     Pagination:  935-45     Citation Subset:  IM    
Laboratory for Cryobiology and Biophysics, Biomedical Research Institute, Rockville, Maryland 20852, USA.
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MeSH Terms
Blood Preservation / methods*
Cell Survival / drug effects
Cryopreservation / methods*
Cryoprotective Agents / chemistry,  pharmacology
Reg. No./Substance:
0/Cryoprotective Agents

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