Document Detail


Cryopreservation of human teeth for future organization of a tooth bank--a preliminary study.
MedLine Citation:
PMID:  16297377     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study was aimed at evaluating whether cryopreserved teeth can be used for future transplantation by examining the viability and differentiation capability of periodontal ligament (PDL) cells and measuring the hardness of dental hard tissue. Fifty-four teeth were divided into two groups, control and frozen teeth. A MTT assay and a TUNEL assay were performed for the examination of the viability and apoptotic death of PDL cells. Immunohistochemical staining for alkaline phosphatase was performed to observe whether the differentiation capability of PDL cells was maintained by the freezing and thawing procedure. Hardness was measured to detect whether dental hard tissue was affected by the freezing conditions. The MTT and TUNEL assays showed no significant difference in the viability of PDL cells between the two groups. The differentiation capability of PDL cells was maintained in frozen teeth as evidenced by alkaline phosphatase staining. The hardness of frozen teeth was not changed, but a longitudinal fracture was found in 25% of the frozen group. The viability and differentiation capability of PDL cells were maintained in a frozen environment; however, it is thought that a new cryopreservation method preventing fracture of dental hard tissue should be developed for clinical application.
Authors:
Young Hak Oh; Zhong Min Che; Jun Chul Hong; Eun Ju Lee; Seung Jong Lee; Jin Kim
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Publication Detail:
Type:  In Vitro; Journal Article     Date:  2005-11-15
Journal Detail:
Title:  Cryobiology     Volume:  51     ISSN:  0011-2240     ISO Abbreviation:  Cryobiology     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-12-12     Completed Date:  2006-03-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  322-9     Citation Subset:  IM    
Affiliation:
Department of Oral Pathology, Oral Cancer Research Institute, Brain Korea 21 Project for Medical Sciences, Seoul 120-749, Republic of Korea.
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MeSH Terms
Descriptor/Qualifier:
Alkaline Phosphatase / metabolism
Apoptosis
Bicuspid / cytology,  physiology,  transplantation
Cell Differentiation
Cell Survival
Cryopreservation / methods*
Hardness Tests
Humans
Periodontal Ligament / cytology,  physiology,  transplantation
Tissue Banks
Tooth* / cytology,  physiology,  transplantation
Chemical
Reg. No./Substance:
EC 3.1.3.1/Alkaline Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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