Document Detail


Cryopreservation of human ovarian tissue.
MedLine Citation:
PMID:  16732415     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
New and often aggressive treatment schemes allow the successful healing of many young patients with cancer, but the price the young women have to pay is high: many of them lose ovarian function and fertility. Due to the improved long-term survival of adolescents and young women with malignancies undergoing gonadotoxic chemotherapy, preservation of future fertility has been the focus of recent ubiquitarian interest. A feasible solution is the cryopreservation of ovarian tissue. Ovarian tissue, after thawing, can be used in three different ways: 1. grafted into its normal site (orthotopic); 2. grafted into a site other than its normal position (heterotopic), necessitating recourse to in vitro fertilization (IVF); 3. grown and in vitro matured in order to obtain metaphase II oocytes for an IVF program. It is believed that protein supplementation, in cryopreservation solution, is essential for improving ovarian tissue cryopreservation. The aim of this study was to evaluate the ultrastructural appearance of human ovarian tissue cryopreserved in 1.5 M 1,2 propanediol (PROH), 0.2 M sucrose using different protein sources: fetal calf serum (FCS), plasmanate or syntetic serum substitute (SSS). Fresh and frozen/thawed ovarian tissues were compared by transmission electron microscope (TEM), to evaluate the appearance of stromal and follicle cells as affected by different protein sources. Our data indicate that FCS is a better protein support for ovarian tissue cryopreservation when compared to SSS or Plasmanate. In addition the follicles are more resistant to the cryopreservation with respect to stroma.
Authors:
Raffaella Fabbri; Gianandrea Pasquinelli; Graziella Bracone; Catia Orrico; Barbara Di Tommaso; Stefano Venturoli
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Cell and tissue banking     Volume:  7     ISSN:  1389-9333     ISO Abbreviation:  Cell Tissue Bank     Publication Date:  2006  
Date Detail:
Created Date:  2006-05-29     Completed Date:  2006-08-18     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  100965121     Medline TA:  Cell Tissue Bank     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  123-33     Citation Subset:  IM    
Affiliation:
Human Reproduction Medicine Unit, S. Orsola-Malpighi Hospital, University of Bologna, Italy. raffaella.fabbri@unibo.it
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Animals
Blood Proteins / metabolism
Cattle
Cell Culture Techniques
Cells, Cultured
Cryopreservation / methods*
Cryoprotective Agents / pharmacology
Female
Fertility
Humans
Ovarian Follicle / cytology,  drug effects,  ultrastructure*
Ovarian Neoplasms / drug therapy
Ovary / cytology*,  drug effects,  ultrastructure
Plasma Substitutes / metabolism
Propylene Glycol / pharmacology
Serum Albumin
Serum Albumin, Bovine / metabolism
Serum Globulins
Stromal Cells / cytology,  drug effects,  ultrastructure*
Sucrose / pharmacology
Chemical
Reg. No./Substance:
0/Blood Proteins; 0/Cryoprotective Agents; 0/Plasma Substitutes; 0/Serum Albumin; 0/Serum Albumin, Bovine; 0/Serum Globulins; 55963-80-9/plasma protein fraction; 57-50-1/Sucrose; 57-55-6/Propylene Glycol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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