| Cryopreservation of human ovarian tissue. | |
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MedLine Citation:
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PMID: 16732415 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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New and often aggressive treatment schemes allow the successful healing of many young patients with cancer, but the price the young women have to pay is high: many of them lose ovarian function and fertility. Due to the improved long-term survival of adolescents and young women with malignancies undergoing gonadotoxic chemotherapy, preservation of future fertility has been the focus of recent ubiquitarian interest. A feasible solution is the cryopreservation of ovarian tissue. Ovarian tissue, after thawing, can be used in three different ways: 1. grafted into its normal site (orthotopic); 2. grafted into a site other than its normal position (heterotopic), necessitating recourse to in vitro fertilization (IVF); 3. grown and in vitro matured in order to obtain metaphase II oocytes for an IVF program. It is believed that protein supplementation, in cryopreservation solution, is essential for improving ovarian tissue cryopreservation. The aim of this study was to evaluate the ultrastructural appearance of human ovarian tissue cryopreserved in 1.5 M 1,2 propanediol (PROH), 0.2 M sucrose using different protein sources: fetal calf serum (FCS), plasmanate or syntetic serum substitute (SSS). Fresh and frozen/thawed ovarian tissues were compared by transmission electron microscope (TEM), to evaluate the appearance of stromal and follicle cells as affected by different protein sources. Our data indicate that FCS is a better protein support for ovarian tissue cryopreservation when compared to SSS or Plasmanate. In addition the follicles are more resistant to the cryopreservation with respect to stroma. |
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Authors:
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Raffaella Fabbri; Gianandrea Pasquinelli; Graziella Bracone; Catia Orrico; Barbara Di Tommaso; Stefano Venturoli |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Cell and tissue banking Volume: 7 ISSN: 1389-9333 ISO Abbreviation: Cell Tissue Bank Publication Date: 2006 |
Date Detail:
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Created Date: 2006-05-29 Completed Date: 2006-08-18 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 100965121 Medline TA: Cell Tissue Bank Country: Netherlands |
Other Details:
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Languages: eng Pagination: 123-33 Citation Subset: IM |
Affiliation:
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Human Reproduction Medicine Unit, S. Orsola-Malpighi Hospital, University of Bologna, Italy. raffaella.fabbri@unibo.it |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Animals Blood Proteins / metabolism Cattle Cell Culture Techniques Cells, Cultured Cryopreservation / methods* Cryoprotective Agents / pharmacology Female Fertility Humans Ovarian Follicle / cytology, drug effects, ultrastructure* Ovarian Neoplasms / drug therapy Ovary / cytology*, drug effects, ultrastructure Plasma Substitutes / metabolism Propylene Glycol / pharmacology Serum Albumin Serum Albumin, Bovine / metabolism Serum Globulins Stromal Cells / cytology, drug effects, ultrastructure* Sucrose / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Blood Proteins; 0/Cryoprotective Agents; 0/Plasma Substitutes; 0/Serum Albumin; 0/Serum Albumin, Bovine; 0/Serum Globulins; 55963-80-9/plasma protein fraction; 57-50-1/Sucrose; 57-55-6/Propylene Glycol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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