Document Detail

Cryopreservation of the human ovarian tissue induces the expression of Fas system in morphologically normal primordial follicles.
MedLine Citation:
PMID:  20687453     Owner:  NLM     Status:  MEDLINE    
The aim of this study was to investigate whether the Fas system was involved in cryopreservation process of the human ovarian tissue. Human ovarian cortical tissues were cryopreserved using slow freezing method. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) was used to investigate apoptosis of primordial follicle. Fas, Fas ligand and active caspase-3 expression were detected by means of immunohistochemistry. The results showed that immunostaining for Fas, Fas ligand and active caspase-3 were not detected in morphologically normal primordial follicles in fresh ovarian tissue. After cryopreservation, Fas, Fas ligand and active caspase-3 immunostaining were present in morphologically normal primordial follicles. The study showed that cryopreservation of the human ovarian tissue induced the expression of Fas system in morphologically normal primordial follicles.
Zhun Xiao; Yan Wang; Lei Li; Shang-Wei Li
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cryo letters     Volume:  31     ISSN:  0143-2044     ISO Abbreviation:  Cryo Letters     Publication Date:    2010 Mar-Apr
Date Detail:
Created Date:  2010-08-06     Completed Date:  2010-09-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9891832     Medline TA:  Cryo Letters     Country:  England    
Other Details:
Languages:  eng     Pagination:  112-9     Citation Subset:  IM    
Reproductive Medical Center of West China 2nd University Hospital, Sichuan University, ChengDu 610041, China.
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MeSH Terms
Antigens, CD95 / metabolism*
Caspase 3 / metabolism
Fas Ligand Protein / metabolism*
In Situ Nick-End Labeling
Ovarian Follicle / cytology,  metabolism*
Young Adult
Reg. No./Substance:
0/Antigens, CD95; 0/FAS protein, human; 0/Fas Ligand Protein; EC 3.4.22.-/Caspase 3

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