Document Detail


Cryopreservation of human hepatocytes for clinical use.
MedLine Citation:
PMID:  20645048     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cryopreservation of hepatocytes is important for use both in research and for clinical application in hepatocyte transplantation. Cryopreservation causes damage to hepatocytes with the result that cell viability and function is reduced on thawing compared to fresh cells. There are many different protocols reported for freezing human hepatocytes mainly using DMSO as cryoprotectant. In this chapter the current detailed protocols used for cryopreservation and thawing of human hepatocytes for cell transplantation at the Cell Isolation Unit at King's College Hospital, London, are described. All procedures must be performed in a clean GMP environment using materials and reagents which are of pharmaceutical grade. The cryopreservation media is UW solution with added 300 mM glucose containing 10% DMSO and the thawing solution is EMEM containing 2% HSA. Freezing is performed in a controlled-rate freezer using a stepwise cooling programme.
Authors:
Ragai R Mitry; Sharon C Lehec; Robin D Hughes
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  640     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2010  
Date Detail:
Created Date:  2010-07-20     Completed Date:  2010-10-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  107-13     Citation Subset:  IM    
Affiliation:
Institute of Liver Studies, King's College Hospital and King's College London School of Medicine, London, UK.
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MeSH Terms
Descriptor/Qualifier:
Adenosine
Allopurinol
Cell Survival
Cryopreservation / methods*
Cryoprotective Agents
Dimethyl Sulfoxide
Glucose
Glutathione
Hepatocytes / cytology*,  transplantation
Humans
Insulin
Organ Preservation Solutions
Raffinose
Serum Albumin
Chemical
Reg. No./Substance:
0/Cryoprotective Agents; 0/Organ Preservation Solutions; 0/Serum Albumin; 0/University of Wisconsin-lactobionate solution; 11061-68-0/Insulin; 315-30-0/Allopurinol; 50-99-7/Glucose; 512-69-6/Raffinose; 58-61-7/Adenosine; 67-68-5/Dimethyl Sulfoxide; 70-18-8/Glutathione

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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