Document Detail


Cryopreservation of human heart cells.
MedLine Citation:
PMID:  3197434     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Single cells were isolated from human heart specimens and preserved at -56 degrees C in a cryoprotecting solution containing dimethyl sulfoxide. The cells were reanimated by rapid thawing and the properties of the cells were evaluated in a physiological solution. The reanimated cells showed morphological and physiological properties similar to those seen in normal, freshly isolated cells. These results demonstrate the feasibility of storing human heart cells for long-term studies.
Authors:
J O Bustamante; D Jachimowicz
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cryobiology     Volume:  25     ISSN:  0011-2240     ISO Abbreviation:  Cryobiology     Publication Date:  1988 Oct 
Date Detail:
Created Date:  1989-01-24     Completed Date:  1989-01-24     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  394-408     Citation Subset:  IM    
Affiliation:
Dalhousie University, Faculty of Medicine, Department of Physiology and Biophysics, Halifax, Nova Scotia, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Separation
Cold Temperature*
Guinea Pigs
Heart / physiology*
Humans
Membrane Potentials
Myocardium / cytology*
Tissue Preservation / methods*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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