Document Detail


Cryopreservation of fetal skin is improved by extracellular trehalose.
MedLine Citation:
PMID:  12237087     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, we tested a non-permeating cryoprotectant, trehalose, in combination with dimethyl sulfoxide (Me(2)SO) in the cryopreservation of human fetal skin and compared it to Me(2)SO and glycerol, protocols that are routinely used by skin banks. The viability of fetal skin from four groups (fresh, and cryopreserved with glycerol, Me(2)SO, or trehalose/Me(2)SO) were evaluated using an in vitro membrane integrity assay and by transplantation to immunodeficient mice. The membrane integrity assay showed a 90% integrity in fresh, unfrozen fetal skin. The number of intact cells dropped to 23 and 44% in fetal skin cryopreserved with glycerol and Me(2)SO, respectively. When trehalose was added to the cryopreservation medium containing Me(2)SO, the membrane integrity rose to 65%. When transplanted to immunodeficient mice, fetal skin cryopreserved with trehalose/Me(2)SO showed a graft performance indistinguishable from fresh unfrozen fetal skin and strikingly better graft take than that of fetal skin cryopreserved with Me(2)SO or glycerol only. These results suggest that cryopreservation protocols routinely used the skin banks can be improved by combining sugars such as trehalose with a permeating cryoprotectant.
Authors:
Gulsun Erdag; Ali Eroglu; Jeffrey Morgan; Mehmet Toner
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article    
Journal Detail:
Title:  Cryobiology     Volume:  44     ISSN:  0011-2240     ISO Abbreviation:  Cryobiology     Publication Date:  2002 Jun 
Date Detail:
Created Date:  2002-09-18     Completed Date:  2003-05-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  218-28     Citation Subset:  IM    
Affiliation:
Center for Engineering in Medicine and Surgical Services, Massachusetts General Hospital and Harvard Medical School, Shriners Burns Hospital, Boston, MA 02114, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Survival
Cryopreservation / methods*
Cryoprotective Agents*
Dimethyl Sulfoxide
Female
Glycerol
Humans
Mice
Mice, SCID
Skin* / anatomy & histology,  cytology,  growth & development
Skin Transplantation
Tissue Preservation / methods*
Transplantation, Heterologous
Trehalose*
Chemical
Reg. No./Substance:
0/Cryoprotective Agents; 56-81-5/Glycerol; 67-68-5/Dimethyl Sulfoxide; 99-20-7/Trehalose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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