| Cryopreservation of the endangered mahseer (Tor khudree) spermatozoa: I. Effect of extender composition, cryoprotectants, dilution ratio, and storage period on post-thaw viability. | |
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MedLine Citation:
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PMID: 15351686 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Several in situ and ex situ conservation strategies have been suggested for the revival of stocks of Tor khudree (Sykes), a threatened species. Cryopreservation of spermatozoa is crucial for the conservation of stocks of endangered species so that sustainable production can be ensured. Among the different extenders, modified fish Ringer (E1) was found to be the best for cryopreservation of T. khudree spermatozoa. Extender E2 appeared the next best. Extenders based on chicken egg yolk and milk powder were found to be unsuitable for the cryopreservation of T. khudree spermatozoa. Among the cryoprotectants, dimethyl sulfoxide provided maximum protection to spermatozoa during freezing and thawing. Propylene glycol and methanol were found to be less effective. Of the four spermatozoa dilutions, 1:10, 1:15, and 1:20 showed better motility rates than 1:5. At the former dilution ratios, the motility rates which were more than 95% prior to freezing were reduced to 80-81 and 43-67%, 10 and 70 days after cryopreservation, respectively. The motility duration did not differ much with increasing storage period at all the dilution ratios. Motility rates generally decreased with an increase in frozen storage. When spermatozoa were thawed and stored at 25 degrees C for varying periods, motility percentage, and duration decreased gradually as the storage period increased; spermatozoa stored up to 40 min after thawing retained 55% motility and were motile up to 77s; these values declined further leading to the complete cessation of motility 70 min after storage. The importance of extender-cryoprotectant mixture, milt dilution, and storage period in developing a protocol for T. khudree spermatozoa cryopreservation is discussed. |
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Authors:
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N Basavaraja; S N Hegde |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cryobiology Volume: 49 ISSN: 0011-2240 ISO Abbreviation: Cryobiology Publication Date: 2004 Oct |
Date Detail:
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Created Date: 2004-09-07 Completed Date: 2005-03-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0006252 Medline TA: Cryobiology Country: United States |
Other Details:
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Languages: eng Pagination: 149-56 Citation Subset: IM |
Affiliation:
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College of Fisheries, University of Agricultural Sciences, Mangalore 575 002, India. basavarajan2002@hotmail.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Survival Cryopreservation / methods* Cryoprotective Agents Cyprinidae* Male Semen Preservation / methods* Solutions Sperm Motility Spermatozoa* / cytology Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Cryoprotective Agents; 0/Solutions |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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