Document Detail


Cryopreservation for corneal storage.
MedLine Citation:
PMID:  19494637     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Currently, cryopreservation is the only method that offers the prospect of truly long-term storage of living cells and tissues. Despite some successful cryopreserved corneal grafts, freezing has been shown to damage the endothelium. When isolated cells are frozen, there are two principal mechanisms of damage: intracellular freezing, which occurs at high cooling rates, and solution effect injury at low cooling rates. When tissues are frozen, there are additional factors that appear to render cells more susceptible to intracellular freezing. Lower cooling rates appear to overcome this when freezing cornea. Vitrification is a way of achieving ice-free cryopreservation, but it also poses considerable challenges owing to the very high solute concentrations required to achieve vitrification at practicable cooling rates. Encouraging results have also been reported for cornea frozen using non-permeating cryoprotectants, which could lead to simpler methods of corneal cryopreservation.
Authors:
W John Armitage
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Publication Detail:
Type:  Journal Article; Review     Date:  2009-06-03
Journal Detail:
Title:  Developments in ophthalmology     Volume:  43     ISSN:  0250-3751     ISO Abbreviation:  Dev Ophthalmol     Publication Date:  2009  
Date Detail:
Created Date:  2009-06-04     Completed Date:  2009-08-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8010321     Medline TA:  Dev Ophthalmol     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  63-9     Citation Subset:  IM    
Copyright Information:
Copyright 2009 S. Karger AG, Basel.
Affiliation:
Department of Clinical Science, University of Bristol, Bristol, UK. w.j.armitage@bristol.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Cornea*
Corneal Transplantation
Cryopreservation / methods*
Cryoprotective Agents
Eye Banks / methods*
Humans
Organ Preservation / methods*
Chemical
Reg. No./Substance:
0/Cryoprotective Agents

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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