Document Detail


Cryopreservation of cells.
MedLine Citation:
PMID:  18432659     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Laboratories interested in working with in vitro cultured cells must be able to freeze and thaw cell lines. Such capabilities are necessary to maintain the constancy of the cells since prolonged culture may result in phenotypic and genotypic changes. In addition, cell freezing minimizes problems with contamination as well as the overall expense of maintaining cell lines for long periods. Finally, freezing of cells provides a method of making cell lines available to other laboratories since frozen cells are readily shipped to distant points. This appendix describes how cell lines can be frozen for long-term storage and thawed for growth in a tissue culture vessel.
Authors:
W M Yokoyama
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Current protocols in immunology / edited by John E. Coligan ... [et al.]     Volume:  Appendix 3     ISSN:  1934-368X     ISO Abbreviation:  Curr Protoc Immunol     Publication Date:  2001 May 
Date Detail:
Created Date:  2008-04-24     Completed Date:  2008-09-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9101651     Medline TA:  Curr Protoc Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  Appendix 3G     Citation Subset:  IM    
Affiliation:
University of California School of Medicine, San Francisco, California, USA.
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MeSH Terms
Descriptor/Qualifier:
Cell Line
Cryopreservation / methods*
Cytological Techniques / methods*
Hybridomas

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