Document Detail


Cryopreservation of cell-containing poly(ethylene) glycol hydrogel microarrays.
MedLine Citation:
PMID:  15932288     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Here we describe the fabrication and preservation of mammalian cell-containing hydrogel microarrays that have potential applications in drug screening and pathogen detection. Hydrogel microstructures containing murine fibroblasts were fabricated on silicon substrates and subjected to a "stage-down" freezing process. The percent viability of both immortal and primary embryonic murine fibroblast cells within the gels was determined at various stages in the freezing process, showing that cells entrapped in hydrogel microstructures remained viable throughout the process. When compared to immortalized adherent cultures subjected to the same freezing process, cells within hydrogel structures had higher cell viabilities at all stages during preservation. Finally, the necessity of using a cryoprotectant, dimethyl sulfoxide (DMSO), was investigated. Cells in hydrogels were cryopreserved with and without DMSO. The addition of DMSO altered cell viability after the freeze-thaw process, enhancing viability in an immortalized cell line and decreasing viability in a primary cell line.
Authors:
Laura J Itle; Michael V Pishko
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology progress     Volume:  21     ISSN:  8756-7938     ISO Abbreviation:  Biotechnol. Prog.     Publication Date:    2005 May-Jun
Date Detail:
Created Date:  2005-06-03     Completed Date:  2005-09-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8506292     Medline TA:  Biotechnol Prog     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1004-7     Citation Subset:  IM    
Affiliation:
The Huck Institute for the Life Sciences and the Department of Chemical Engineering, The Pennsylvania State University, University Park, Pennsylvania 16802-4400, USA.
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MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Animals
Biological Assay / instrumentation,  methods*
Cell Culture Techniques / instrumentation,  methods*
Cell Proliferation
Cell Survival
Cryopreservation / instrumentation,  methods*
Equipment Design
Equipment Failure Analysis
Hydrogels / chemistry
Mice
Microarray Analysis / instrumentation,  methods*
Microfluidic Analytical Techniques / instrumentation,  methods*
Polyethylene Glycols / chemistry*
Chemical
Reg. No./Substance:
0/Hydrogels; 0/Polyethylene Glycols

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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