Document Detail


Cryopreservation of carrot (Daucus carota l.) cell suspensions and protoplasts by vitrification.
MedLine Citation:
PMID:  12644854     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Carrot cell suspensions and protoplasts were successfully cryopreserved by vitrification. Cells were precultured in liquid Murashige and Skoog medium containing 0.175 M sucrose for 3 d and then in liquid MS medium containing 0.4 M sorbitol for 1 d. After loading of the precultured carrot cells in 25 % PVS2 at room temperature for 5 min and treatment with 100 % PVS2 at 0 degrees C for 7.5 min, they were quenched in liquid nitrogen. Optimal survival was 83.3 % (based on the triphenyl tetrazolium chloride reduction assay) following warming and unloading. Recovered cells retained the ability to regenerate plantlets in vitro. In the case of vitrification of protoplasts isolated from carrot cell suspensions, the optimal loading and dehydration durations were 5 min in 25% PVS2 and 3 min 100 % PVS2 respectively. Survival of 47 % of the untreated control (based on the FDA-PI (fluorescein diacetate-propidium iodide) staining) was achieved after cryopreservation.
Authors:
Yong Chen; Jun-Hui Wang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cryo letters     Volume:  24     ISSN:  0143-2044     ISO Abbreviation:  Cryo Letters     Publication Date:    2003 Jan-Feb
Date Detail:
Created Date:  2003-03-19     Completed Date:  2003-07-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9891832     Medline TA:  Cryo Letters     Country:  England    
Other Details:
Languages:  eng     Pagination:  57-64     Citation Subset:  IM    
Affiliation:
Department of Biological and Environmental Sciences, Wenzhou Normal College, Wenzhou 325003, China.
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MeSH Terms
Descriptor/Qualifier:
Cryopreservation*
Daucus carota / cytology*
Protoplasts

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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