Document Detail

Cryopreservation based on freezing protocols for the long-term storage of microencapsulated myoblasts.
MedLine Citation:
PMID:  19327822     Owner:  NLM     Status:  MEDLINE    
One important challenge in biomedicine is the ability to cryogenically preserve not only cells, but also tissue-engineered constructs. In the present paper, alginate-poly-l-lysine-alginate (APA) microcapsules containing erythropoietin (Epo)-secreting C(2)C(12) myoblasts were elaborated, characterized and tested both in vitro and in vivo. Dimethylsulfoxide (DMSO) was selected as cryoprotectant to evaluate the maintenance of physiological activity of cryopreserved microencapsulated myoblasts employing procedures based on freezing protocols up to a 45-day cryopreservation period. High chemical resistance of the cryopreserved microcapsules was observed using 10% DMSO as cryoprotectant following a standard slow-cooling procedure. Although a 42% reduction in Epo release from the microencapsulated cells was observed in comparison with the non-cryopreserved group, the in vivo biocompatibility and functionality of the encapsulated cells subcutaneously implanted in Balb/c mice was corroborated by high and sustained hematocrit levels over 194 days and lacking immunosuppressive protocols. No major host reaction was observed. Based on the results obtained in our study, a slow-cooling protocol using 10% DMSO as cryoprotectant (confirmed for cryopreservation periods up to 45 days) might be considered a suitable therapeutic strategy if the long-term storage of microencapsulated cells, such as C(2)C(12) myoblasts is pretended.
Ainhoa Murua; Gorka Orive; Rosa M Hernández; José Luis Pedraz
Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-03-27
Journal Detail:
Title:  Biomaterials     Volume:  30     ISSN:  1878-5905     ISO Abbreviation:  Biomaterials     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-05-15     Completed Date:  2009-06-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  3495-501     Citation Subset:  IM    
University of the Basque Country, Vitoria-Gasteiz, Spain.
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MeSH Terms
Alginates / chemistry*,  metabolism
Biocompatible Materials / chemistry,  metabolism
Capsules / chemistry*
Cell Line
Cryopreservation / methods*
Cryoprotective Agents / metabolism
Dimethyl Sulfoxide / metabolism
Erythropoietin / metabolism
Materials Testing
Mice, Inbred BALB C
Myoblasts / cytology,  physiology*,  transplantation*
Osmotic Pressure
Polylysine / analogs & derivatives*,  chemistry,  metabolism
Transplantation, Homologous
Reg. No./Substance:
0/Alginates; 0/Biocompatible Materials; 0/Capsules; 0/Cryoprotective Agents; 0/alginate-polylysine-alginate; 11096-26-7/Erythropoietin; 25104-18-1/Polylysine; 67-68-5/Dimethyl Sulfoxide

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