Document Detail

Cryopreservation of anoxygenic phototrophic Fe(II)-oxidizing bacteria.
MedLine Citation:
PMID:  20433824     Owner:  NLM     Status:  MEDLINE    
Preservation and storage of microbial stock cultures is desirable since the risk of contamination or loss of living cultures is immanent while over long periods mutations accumulate. Generally, it is rather difficult to preserve photosynthetic bacteria due to their sensitive photosynthetic membranes. Phototrophic Fe(II)-oxidizing bacteria face an additional challenge; since they are exposed to light and Fe(II) during growth, they have to cope with radicals from Fenton reactions of Fe(II)-species, light and water. Therefore, phototrophic Fe(II)-oxidizing strains are thought to be especially susceptible to genetic modifications. Here, we provide a simple and fast protocol using glycerol as cryo-protectant to cryopreserve three strains of anoxygenic phototrophic Fe(II)-oxidizing bacteria from different taxa: alpha-proteobacteria, gamma-proteobacteria and chloroflexi. All three strains investigated could be revived after 17 months at -72 degrees C. This suggests that a long-term storage of phototrophic Fe(II)-oxidizing strains is possible.
F Hegler; A Kappler
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-04-28
Journal Detail:
Title:  Cryobiology     Volume:  61     ISSN:  1090-2392     ISO Abbreviation:  Cryobiology     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-08-09     Completed Date:  2010-11-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  158-60     Citation Subset:  IM    
Copyright Information:
(c) 2010 Elsevier Inc. All rights reserved.
Geomicrobiology, Center for Applied Geosciences, University of Tuebingen, Germany.
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MeSH Terms
Bacteria / cytology,  drug effects*,  metabolism
Cryopreservation / methods*
Cryoprotective Agents / pharmacology*
Ferrous Compounds / metabolism*
Glycerol / pharmacology*
Reg. No./Substance:
0/Cryoprotective Agents; 0/Ferrous Compounds; 56-81-5/Glycerol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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