Document Detail


Cryopreservation of animal and human cell lines.
MedLine Citation:
PMID:  18080474     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The long-term storage of human and animal cell lines is a relatively new branch of the science of cryopreservation of living organisms. The need to maintain the increasing numbers of cell lines, as they started to emerge from research laboratories from the mid-1900s, necessitated a radical approach to the problem. The realization that they could survive cryopreservation, and that a slow rate of cooling is essential for this survival, led to the eventual discovery of cryoprotectants. Subsequent development of mechanical freezers, which can accurately control the rate of cooling, now allows cells to be cryopreserved at their maximum viability. This chapter outlines the essentials steps for the successful preparation, freezing, and storage of cell lines.
Authors:
Christopher B Morris
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Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  368     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2007  
Date Detail:
Created Date:  2007-12-17     Completed Date:  2008-07-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  227-36     Citation Subset:  IM    
Affiliation:
European Collection of Animal Cell Cultures, Health Protection Agency, Centre for Emergency Preparedness and Response, Salisbury, Wiltshire, UK.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line*
Cell Survival
Cryopreservation* / methods
Cryoprotective Agents*
Humans
Chemical
Reg. No./Substance:
0/Cryoprotective Agents

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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