Document Detail

Cryopreservation of Taxus chinensis suspension cell cultures.
MedLine Citation:
PMID:  11788843     Owner:  NLM     Status:  MEDLINE    
A simple cryopreservation method for suspension cells of Taxus chinensis was established. In this procedure 7 days old suspension cells were used without any pre-culture treatment. At first, cells were incubated in cryoprotectant solution (0.5M DMSO and 0.5M glycerol) on ice for 30 min and then frozen at a cooling rate of 1 degree C/min to -40 degrees C prior to immersion in liquid nitrogen. The average viability of frozen-thawed cells was between 30 to 40%. The recovery of cryopreserved cells in liquid nitrogen for 1 month was accomplished. After rapid thawing, cells were transferred to solid medium and cultivated for 4-6 weeks. The treatment of trehalose as a cryoprotectant enhanced re-growth of frozen-thawed cells. The stable maintenance of paclitaxel biosynthetic ability in cryopreserved cells was confirmed by comparing with that of regularly sub-cultured suspension cells.
S I Kim; H K Choi; J S Son; J H Yun; M S Jang; H R Kim; J Y Song; J H Kim; H J Choi; S S Hong
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cryo letters     Volume:  22     ISSN:  0143-2044     ISO Abbreviation:  Cryo Letters     Publication Date:    2001 Jan-Feb
Date Detail:
Created Date:  2002-01-25     Completed Date:  2002-02-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9891832     Medline TA:  Cryo Letters     Country:  England    
Other Details:
Languages:  eng     Pagination:  43-50     Citation Subset:  IM    
Samyang Genex Biotech Research Institute,63-2, Hwaam-Dong, Yusung-Gu, Taejon, 305-348, Korea.
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MeSH Terms
Cell Survival
Cells, Cultured
Cryoprotective Agents
Taxus / cytology*
Reg. No./Substance:
0/Cryoprotective Agents

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