Document Detail

Cryopreservation of Crassostrea gigas vesicular cells: viability and metabolic activity.
MedLine Citation:
PMID:  16698009     Owner:  NLM     Status:  MEDLINE    
Cryopreservation is widely used for long-term conservation of various tissues, embryos or gametes. However, few studies have described cryopreservation of invertebrate primary cell cultures and more particularly of marine invertebrate somatic cells. This technique would however be of great interest to facilitate the study of various metabolic processes which vary seasonally. The aim of the present study was to develop a protocol for cryopreservation of Crassostrea gigas vesicular cells. Different parameters were adjusted to improve recovery of cells after freezing. The most efficient cryoprotectant agent was a mix of Me(2)SO, glycerol, and ethylene glycol (4% each). The optimal cooling rate was -1 degrees Cmin(-1) down to -70 degrees C before transfer into liquid nitrogen. In these conditions the percentage of viable cells reached 70% of the control. The glucose metabolism of thawed cells was evaluated using radioactive glucose as a tracer. Immediately after thawing, glucose uptake involving membrane transporters was greatly reduced (24% of control) whereas glucose incorporation into glycogen was less affected (68% of control).
A C Hanquet-Dufour; K Kellner; C Heude; A Naimi; M Mathieu; J M Poncet
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-05-15
Journal Detail:
Title:  Cryobiology     Volume:  53     ISSN:  0011-2240     ISO Abbreviation:  Cryobiology     Publication Date:  2006 Aug 
Date Detail:
Created Date:  2006-07-17     Completed Date:  2006-09-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  28-36     Citation Subset:  IM    
UMR PE2M, Laboratoire de Biologie et Biotechnologies Marines, UMR 100 Ifremer, Physiologie et Ecophysiologie des Mollusques Marins, Université de Caen, Esplanade de la Paix, F 14032 Caen Cedex, France.
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MeSH Terms
Cell Survival
Crassostrea / cytology*,  metabolism
Cryopreservation / methods*
Glucose / metabolism
Glycogen / biosynthesis
Tetrazolium Salts / metabolism
Thiazoles / metabolism
Reg. No./Substance:
0/Tetrazolium Salts; 0/Thiazoles; 298-93-1/thiazolyl blue; 50-99-7/Glucose; 9005-79-2/Glycogen

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