| Cryopreservation of Chrysanthemum morifolium (Dendranthema grandiflora Ramat.) using different approaches. | |
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MedLine Citation:
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PMID: 13680136 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Chrysanthemum species are grown both as ornamentals and for the production of pyrethrum. Recent increased production and breeding efforts have raised the need for the conservation of valuable germplasm. Chrysanthemum has been cryopreserved by controlled-rate-freezing as early as 1990. We report here deep-freezing of shoot tips of C. morifolium var. Escort by different technical procedures: controlled-rate-freezing, encapsulation/dehydration, ultra-rapid-freezing by the droplet method and vitrification. While vitrification yielded the highest shoot regeneration rates, the very simple droplet method was also successful in this respect. Droplet freezing was successfully performed with nine cultivars. Our results open the door to the successful use of alternative methods if one method fails to cryopreserve a variety. Furthermore, it enables comparative investigations of genetic stability and cyro-injury to be carried out. |
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Authors:
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A Halmagyi; G Fischer-Klüver; G Mix-Wagner; H M Schumacher |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2003-09-16 |
Journal Detail:
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Title: Plant cell reports Volume: 22 ISSN: 0721-7714 ISO Abbreviation: Plant Cell Rep. Publication Date: 2004 Jan |
Date Detail:
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Created Date: 2003-12-19 Completed Date: 2004-04-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9880970 Medline TA: Plant Cell Rep Country: Germany |
Other Details:
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Languages: eng Pagination: 371-5 Citation Subset: IM |
Affiliation:
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Institute of Biological Research, Cluj-Napoca, Romania. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Chrysanthemum
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cytology*,
physiology Cryopreservation / methods* Freeze Drying Freezing Insecticides Kinetics Plant Shoots / physiology Pyrethrins / metabolism* Regeneration Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Insecticides; 0/Pyrethrins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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