Document Detail

Cryopreservation of Chrysanthemum morifolium (Dendranthema grandiflora Ramat.) using different approaches.
MedLine Citation:
PMID:  13680136     Owner:  NLM     Status:  MEDLINE    
Chrysanthemum species are grown both as ornamentals and for the production of pyrethrum. Recent increased production and breeding efforts have raised the need for the conservation of valuable germplasm. Chrysanthemum has been cryopreserved by controlled-rate-freezing as early as 1990. We report here deep-freezing of shoot tips of C. morifolium var. Escort by different technical procedures: controlled-rate-freezing, encapsulation/dehydration, ultra-rapid-freezing by the droplet method and vitrification. While vitrification yielded the highest shoot regeneration rates, the very simple droplet method was also successful in this respect. Droplet freezing was successfully performed with nine cultivars. Our results open the door to the successful use of alternative methods if one method fails to cryopreserve a variety. Furthermore, it enables comparative investigations of genetic stability and cyro-injury to be carried out.
A Halmagyi; G Fischer-Klüver; G Mix-Wagner; H M Schumacher
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2003-09-16
Journal Detail:
Title:  Plant cell reports     Volume:  22     ISSN:  0721-7714     ISO Abbreviation:  Plant Cell Rep.     Publication Date:  2004 Jan 
Date Detail:
Created Date:  2003-12-19     Completed Date:  2004-04-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9880970     Medline TA:  Plant Cell Rep     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  371-5     Citation Subset:  IM    
Institute of Biological Research, Cluj-Napoca, Romania.
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MeSH Terms
Chrysanthemum / cytology*,  physiology
Cryopreservation / methods*
Freeze Drying
Plant Shoots / physiology
Pyrethrins / metabolism*
Time Factors
Reg. No./Substance:
0/Insecticides; 0/Pyrethrins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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