Document Detail


Cryoelectron tomography of eukaryotic cells.
MedLine Citation:
PMID:  20888478     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Biological processes involve a high degree of protein dynamics resulting in a constant remodeling of the cellular landscape at the molecular level. Orchestrated changes lead to significant rearrangement of the eukaryotic cytoskeleton and nuclear structures. Visualization of the cellular landscape in the unperturbed state is essential for understanding these processes. The development of cryoelectron tomography (cryo-ET) and its application to eukaryotic cells has provided a major step forward toward better realizing these processes. In conjunction with rapid freezing techniques, that is, vitrification by plunge-freezing and high-pressure freezing, cryo-ET is most suitable for investigating cellular ultrastructures in a close-to-life state. Here, we review the application of cryo-ET to the study of eukaryotic cells, with special emphasis on sample preparation, cytoskeleton organization, and macromolecular structures observed at a resolution of 4-6 nm.
Authors:
Asaf Mader; Nadav Elad; Ohad Medalia
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Methods in enzymology     Volume:  483     ISSN:  1557-7988     ISO Abbreviation:  Meth. Enzymol.     Publication Date:  2010  
Date Detail:
Created Date:  2010-10-04     Completed Date:  2011-01-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0212271     Medline TA:  Methods Enzymol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  245-65     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
Affiliation:
Department of Life Sciences, Ben Gurion University of the Negev, Beer-Sheva, Israel.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cryoelectron Microscopy / methods
Cytoskeleton
Dictyostelium / ultrastructure
Electron Microscope Tomography / methods*
Eukaryotic Cells / ultrastructure*
Microscopy, Fluorescence
Nuclear Pore / ultrastructure

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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