Document Detail

Cryoablation of renal cancer: variables involved in freezing-induced cell death.
MedLine Citation:
PMID:  17375969     Owner:  NLM     Status:  MEDLINE    
The detection of renal tumors has increased significantly over recent years resulting in a greater demand for novel, minimally invasive techniques. Cryoablation has emerged as a valuable treatment modality for the management of renal cancer. In an effort to detail the effects of freezing in renal cancer, the human renal cancer (RCC) cell line, 786-O, was evaluated in vitro. 786-O cells were exposed to a range of freezing temperatures from -5 to -40 degrees C and compared to non-frozen controls. The data show that freezing to -5 degrees C did not affect 786-O cell viability, while -10 degrees C, -15 degrees C, and -20 degrees C results in a significant loss of viability (23, 70, and 91%, respectively). A complete loss of cell viability was evident at temperatures of -25 degrees C and colder. Following this analysis, variables involved in the success of cryoablation were investigated. For each of the temperatures tested, extended freeze hold times and passive thawing rates resulted in more extensive cell damage. Additionally, a double freeze-thaw cycle significantly increased cell death compared to a single cycle (62% vs. 22% at -10 degrees C; 89% vs. 63% at -15 degrees C, respectively). While these variables play an important part in the effective application of cryoablation, a molecular understanding of the cell death involved is critical to improving efficacy. Apoptotic inhibition afforded 12% (-10 degrees C), 25% (-15 degrees C), and 11% (-20 degrees C) protection following freezing. Using fluorescence microscopy analysis, the results demonstrated that apoptosis peaked at six hours post-thaw. Next, apoptotic initiating agents including 5-FU and resveratrol (RVT) applied prior to freezing exposure resulted in a significant increase in cell death compared to either application alone. Importantly, the combination of RVT and freezing was noticeably less effective when applied to normal renal cells. The results herein demonstrate the efficacy of freezing and describe a novel therapeutic model for the treatment of renal cancer that may distinguish between cancer and normal cells.
Dominic M Clarke; Anthony T Robilotto; Elisa Rhee; Robert G VanBuskirk; John G Baust; Andrew A Gage; John M Baust
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Technology in cancer research & treatment     Volume:  6     ISSN:  1533-0346     ISO Abbreviation:  Technol. Cancer Res. Treat.     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-03-22     Completed Date:  2007-05-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101140941     Medline TA:  Technol Cancer Res Treat     Country:  United States    
Other Details:
Languages:  eng     Pagination:  69-79     Citation Subset:  IM    
Cell Preservation Services, Inc., Owego, NY 13827, USA.
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MeSH Terms
Cell Death
Cell Line, Tumor
Cell Survival
Cryosurgery / methods*
Kidney Neoplasms / pathology*

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