Document Detail


Cryo-ultramicrotomy as a tool for the assessment of doxorubicine cardiotoxicity in rats.
MedLine Citation:
PMID:  7389974     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cryo-ultramicrotomy was tested as a tool for the assessment of cardiac damage induced by doxorubicine in rats. Slightly fixed specimens were cut at -80 or -90 degrees C (knife temperature -60 or -70 degrees C), and the ultrastructural changes observed were compared with those found in conventional epoxy sections. It is concluded that the cryo method, although using more elaborate equipment and being more painstaking, might yield more sensitive parameters for cardiac damage than epoxy sections.
Authors:
C Holderegger; G Zbinden
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Experimental cell biology     Volume:  48     ISSN:  0304-3568     ISO Abbreviation:  Exp. Cell Biol.     Publication Date:  1980  
Date Detail:
Created Date:  1980-09-28     Completed Date:  1980-09-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7701827     Medline TA:  Exp Cell Biol     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  349-55     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Doxorubicin / toxicity*
Drug Evaluation, Preclinical / methods
Epoxy Resins
Female
Frozen Sections
Heart / drug effects*
Microscopy, Electron
Microtomy / methods*
Mitochondria, Heart / ultrastructure
Myocardium / ultrastructure
Rats
Chemical
Reg. No./Substance:
0/Epoxy Resins; 23214-92-8/Doxorubicin; 67076-53-3/EPON

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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