Document Detail


Cryo-scanning electron microscopic study on freezing behaviors of tissue cells in dormant buds of larch (Larix kaempferi).
MedLine Citation:
PMID:  19646981     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The freezing behavior of dormant buds in larch, especially at the cellular level, was examined by a Cryo-SEM. The dormant buds exhibited typical extraorgan freezing. Extracellular ice crystals accumulated only in basal areas of scales and beneath crown tissues, areas in which only these living cells had thick walls unlike other tissue cells. By slow cooling (5 degrees C/day) of dormant buds to -50 degrees C, all living cells in bud tissues exhibited distinct shrinkage without intracellular ice formation detectable by Cryo-SEM. However, the recrystallization experiment of these slowly cooled tissue cells, which was done by further freezing of slowly cooled buds with LN and then rewarming to -20 degrees C, confirmed that some of the cells in the leaf primordia, shoot primordia and apical meristem, areas in which cells had thin walls and in which no extracellular ice accumulated, lost freezable water with slow cooling to -30 degrees C, indicating ability of these cells to adapt by extracellular freezing, whereas other cells in these tissues retained freezable water with slow cooling even to -50 degrees C, indicating adaptation of these cells by deep supercooling. On the other hand, all cells in crown tissues and in basal areas of scales, areas in which cells had thick walls and in which large masses of ice accumulated, had the ability to adapt by extracellular freezing. It is thought that the presence of two types of cells exhibiting different freezing adaptation abilities within a bud tissue is quite unique and may reflect sophisticated freezing adaptation mechanisms in dormant buds.
Authors:
Keita Endoh; Jun Kasuga; Keita Arakawa; Toshiaki Ito; Seizo Fujikawa
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-07-30
Journal Detail:
Title:  Cryobiology     Volume:  59     ISSN:  1090-2392     ISO Abbreviation:  Cryobiology     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-08-31     Completed Date:  2009-10-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  214-22     Citation Subset:  IM    
Affiliation:
Research Faculty and Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
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MeSH Terms
Descriptor/Qualifier:
Cryoelectron Microscopy
Crystallization
Freezing*
Larix
Microscopy, Electron, Scanning
Plant Shoots / cytology*,  physiology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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