Document Detail

Cryo-injury and biopreservation.
MedLine Citation:
PMID:  16533923     Owner:  NLM     Status:  MEDLINE    
Mammalian cells appear to be naturally tolerant to cold temperatures, but the formation of ice when cells are cooled leads to a variety of damaging effects. The study of cryo-injury, therefore, becomes the study of when and how ice is formed both inside and outside the cell during cooling. Protectant chemicals are used to control or prevent ice formation in many preservation protocols, but these chemical themselves tend to be damaging. Cooling and warming rates also strongly affect the amount and location of ice that is formed. Through careful modification of these parameters successful cold preservation techniques for many cell types have been developed, but there are many more cell types that have defied preservation techniques, and the extension of cell-based techniques to tissues and whole organs has been very limited. There are many aspects to the damaging effects of ice in cells that are still poorly understood. In this brief article we review our current understanding of cellular injury and highlight the aspects of cellular injury during cryopreservation that are still poorly understood.
Alex Fowler; Mehmet Toner
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Review    
Journal Detail:
Title:  Annals of the New York Academy of Sciences     Volume:  1066     ISSN:  0077-8923     ISO Abbreviation:  Ann. N. Y. Acad. Sci.     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2006-03-14     Completed Date:  2006-09-27     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7506858     Medline TA:  Ann N Y Acad Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  119-35     Citation Subset:  IM    
Department of Mechanical Engineering, University of Massachusetts, Dartmouth, MA, USA.
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MeSH Terms
Cell Membrane / pathology
Osmolar Concentration
Reg. No./Substance:

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