Document Detail


Cryo-electron microscopy modeling by the molecular dynamics flexible fitting method.
MedLine Citation:
PMID:  22696404     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The increasing power and popularity of cryo-electron microscopy (cryo-EM) in structural biology brought about the development of so-called hybrid methods, which permit the interpretation of cryo-EM density maps beyond their nominal resolution in terms of atomic models. The Cryo-EM Modeling Challenge 2010 is the first community effort to bring together developers of hybrid methods as well as cryo-EM experimentalists. Participating in the challenge, the molecular dynamics flexible fitting (MDFF) method was applied to a number of cryo-EM density maps. The results are described here with special emphasis on the use of symmetry-based restraints to improve the quality of atomic models derived from density maps of symmetric complexes; on a comparison of the stereochemical quality of atomic models resulting from different hybrid methods; and on application of MDFF to electron crystallography data.
Authors:
Kwok-Yan Chan; Leonardo G Trabuco; Eduard Schreiner; Klaus Schulten
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Biopolymers     Volume:  97     ISSN:  0006-3525     ISO Abbreviation:  Biopolymers     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-06-14     Completed Date:  2012-08-21     Revised Date:  2013-09-03    
Medline Journal Info:
Nlm Unique ID:  0372525     Medline TA:  Biopolymers     Country:  United States    
Other Details:
Languages:  eng     Pagination:  678-86     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
Affiliation:
Department of Physics, University of Illinois, Urbana-Champaign, Urbana, IL 61801, USA.
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MeSH Terms
Descriptor/Qualifier:
Cryoelectron Microscopy / methods*
Crystallography
Molecular Dynamics Simulation*
Stereoisomerism
Grant Support
ID/Acronym/Agency:
P41 RR005969-22/RR/NCRR NIH HHS; P41-RR005969/RR/NCRR NIH HHS
Comments/Corrections

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