| Cryo-electron microscopy modeling by the molecular dynamics flexible fitting method. | |
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MedLine Citation:
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PMID: 22696404 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The increasing power and popularity of cryo-electron microscopy (cryo-EM) in structural biology brought about the development of so-called hybrid methods, which permit the interpretation of cryo-EM density maps beyond their nominal resolution in terms of atomic models. The Cryo-EM Modeling Challenge 2010 is the first community effort to bring together developers of hybrid methods as well as cryo-EM experimentalists. Participating in the challenge, the molecular dynamics flexible fitting (MDFF) method was applied to a number of cryo-EM density maps. The results are described here with special emphasis on the use of symmetry-based restraints to improve the quality of atomic models derived from density maps of symmetric complexes; on a comparison of the stereochemical quality of atomic models resulting from different hybrid methods; and on application of MDFF to electron crystallography data. |
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Authors:
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Kwok-Yan Chan; Leonardo G Trabuco; Eduard Schreiner; Klaus Schulten |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Biopolymers Volume: 97 ISSN: 0006-3525 ISO Abbreviation: Biopolymers Publication Date: 2012 Sep |
Date Detail:
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Created Date: 2012-06-14 Completed Date: 2012-08-21 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 0372525 Medline TA: Biopolymers Country: United States |
Other Details:
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Languages: eng Pagination: 678-86 Citation Subset: IM |
Copyright Information:
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Copyright © 2012 Wiley Periodicals, Inc. |
Affiliation:
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Department of Physics, University of Illinois, Urbana-Champaign, Urbana, IL 61801, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cryoelectron Microscopy
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methods* Crystallography Molecular Dynamics Simulation* Stereoisomerism |
| Grant Support | |
ID/Acronym/Agency:
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P41 RR005969-22/RR/NCRR NIH HHS; P41-RR005969/RR/NCRR NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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