Document Detail

Cryo-electron microscopy modeling by the molecular dynamics flexible fitting method.
MedLine Citation:
PMID:  22696404     Owner:  NLM     Status:  MEDLINE    
The increasing power and popularity of cryo-electron microscopy (cryo-EM) in structural biology brought about the development of so-called hybrid methods, which permit the interpretation of cryo-EM density maps beyond their nominal resolution in terms of atomic models. The Cryo-EM Modeling Challenge 2010 is the first community effort to bring together developers of hybrid methods as well as cryo-EM experimentalists. Participating in the challenge, the molecular dynamics flexible fitting (MDFF) method was applied to a number of cryo-EM density maps. The results are described here with special emphasis on the use of symmetry-based restraints to improve the quality of atomic models derived from density maps of symmetric complexes; on a comparison of the stereochemical quality of atomic models resulting from different hybrid methods; and on application of MDFF to electron crystallography data.
Kwok-Yan Chan; Leonardo G Trabuco; Eduard Schreiner; Klaus Schulten
Related Documents :
22612094 - Rovibrational dynamics of the strontium molecule in the a(1)Σ(u) (+), c(3)Π(u), and a...
6268014 - The presence of intranuclear lipid inclusions in hepatocytes of mice after chronic inge...
24596464 - Direct electrochemical oxidation of s-captopril using gold electrodes modified with gra...
22820044 - Ft-ir and ft-raman spectra, thermo dynamical behavior, homo and lumo, uv, nlo propertie...
186114 - Function of three cytochromes in photosynthesis of whole cells of rhodospirillum rubrum...
11494644 - Energy expenditure, physical activity, and obesity in children.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Biopolymers     Volume:  97     ISSN:  0006-3525     ISO Abbreviation:  Biopolymers     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-06-14     Completed Date:  2012-08-21     Revised Date:  2014-09-08    
Medline Journal Info:
Nlm Unique ID:  0372525     Medline TA:  Biopolymers     Country:  United States    
Other Details:
Languages:  eng     Pagination:  678-86     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cryoelectron Microscopy / methods*
Molecular Dynamics Simulation*
Grant Support
P41 RR005969/RR/NCRR NIH HHS; P41 RR005969-22/RR/NCRR NIH HHS; P41-RR005969/RR/NCRR NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Gorgon and pathwalking: macromolecular modeling tools for subnanometer resolution density maps.
Next Document:  Real-space refinement with DireX: From global fitting to side-chain improvements.