Document Detail

Cryo-TEM reconstructions of Triatoma Virus particles: a clue to unravel genome delivery and capsid disassembly.
MedLine Citation:
PMID:  23288423     Owner:  NLM     Status:  Publisher    
Triatoma Virus (TrV) is a member of the insect virus family Dicistroviridae and consists of a small, non-enveloped capsid that encloses its +ssRNA genome. Using cryo-TEM and 3D reconstruction techniques combined with the fitting of the available crystallographic models, we analyzed the capsids corresponding to mature and several RNA-empty TrV particles. After genome release, the resulting reconstruction of the empty capsids displays no prominent conformational changes with respect to the full virion capsid. Our results show that RNA delivery leads to empty capsids with an apparent overall intact protein shell and suggest that, in a subsequent step, empty capsids disassemble into small symmetrical particles. Contrary to what is observed upon genome release in mammalian picornaviruses, the empty TrV capsid maintains an identical protein shell thickness and size as in full virions.
Jon Agirre; Gael Goret; Melanie Legoff; Rubén Sánchez-Eugenia; Gerardo Marti; Jorge Navaza; Diego Guérin; Emmanuelle Neumann
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-3
Journal Detail:
Title:  The Journal of general virology     Volume:  -     ISSN:  1465-2099     ISO Abbreviation:  J. Gen. Virol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0077340     Medline TA:  J Gen Virol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Unidad de Biofísica, Bilbao, Spain;
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