Document Detail


Crude extract of rheum palmatum L induced cell death in LS1034 human colon cancer cells acts through the caspase-dependent and -independent pathways.
MedLine Citation:
PMID:  23315830     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Crude extract of Rheum palmatum L (CERP) has been used to treat different diseases in the Chinese population for decades. In this study, we investigated the effects of CERP on LS1034 human colorectal cancer cells in vitro and also examined possible mechanisms of cell death. Flow cytometric assays were used to measure the percentage of viable cells, cell cycle distribution including the sub-G1 phase (apoptosis), the activities of caspase-8, -9, and -3, reactive oxygen species (ROS) and Ca(2+) levels, and mitochondrial membrane potential (ΔΨ(m) ). DNA damage, nuclei condensation, protein expression, and translocation were examined by Comet assay, 4'-6-diamidino-2-phenylindole (DAPI) staining, Western blotting, and confocal laser system microscope, respectively. CERP induced apoptosis as seen by DNA fragmentation and DAPI staining in a concentration- and time-dependent manner in cancer cells. CERP was associated with an increase in the Bax/Bcl-2 protein ratio and CERP promoted the activities of caspase-8, -9, and -3. Both ROS and Ca(2+) levels were increased by CERP but the compound decreased levels of ΔΨ(m) in LS1034 cells. Laser confocal microscope also confirmed that CERP promoted the expressions of AIF, Endo G, cytochrome c, and GADD153 to induce apoptosis through mitochondrial-dependent pathway. © 2013 Wiley Periodicals, Inc. Environ Toxicol, 2013.
Authors:
Yi-Shih Ma; Shu-Chun Hsu; Shu-Wen Weng; Chien-Chih Yu; Jai-Sing Yang; Kuang-Chi Lai; Jing-Pin Lin; Jaung-Geng Lin; Jing-Gung Chung
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-12
Journal Detail:
Title:  Environmental toxicology     Volume:  -     ISSN:  1522-7278     ISO Abbreviation:  Environ. Toxicol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100885357     Medline TA:  Environ Toxicol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013 Wiley Periodicals, Inc.
Affiliation:
Graduate Institute of Chinese Medicine, China Medical University, Taichung 404, Taiwan; Department of Chinese Medicine, Changhua Hospital, Department of Health, Executive Yuan, Changhua 513, Taiwan.
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