Document Detail


Crude cacao Theobroma cacao extract reduces mutagenicity induced by benzo[a]pyrene through inhibition of CYP1A activity in vitro.
MedLine Citation:
PMID:  19170136     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Polyphenols have been shown to have potent antioxidant activity, and therefore, food containing polyphenols is expected to contribute to the prevention of cancer. However, food contains not only polyphenols but also various other constituents. We used the Ames test to investigate the effects of crude extracts of whole cacao products, which are known to be rich in polyphenols, on the mutagenicity of benzo[a]pyrene (B[a]P) in Salmonella typhimurium strain TA 98 and tert-butyl hydroperoxide (t-BuOOH) in S. typhimurium strain TA 102. B[a]P induces mutagenicity by metabolic activation and t-BuOOH induces it by generation of free radicals. While white chocolate did not modulate the numbers of revertant colonies produced by B[a]P treatment, milk chocolate and cacao powder extracts did. On the other hand, surprisingly, none of the cacao products tested affected the number of revertant colonies when t-BuOOH was used as the mutagen. At maximum concentration (13.25 mg cacao powder/ml), the crude cacao powder extract reduced ethoxyresorufin O-deethylase activity to 17.4% of the control, suggesting that whole cacao products inhibit cytochrome P450 (CYP) 1A activity. In conclusion, inhibition of CYP1A activity by cacao products may prevent DNA damage by reducing metabolic activation of carcinogens.
Authors:
Marumi Ohno; Kentaro Q Sakamoto; Mayumi Ishizuka; Shoichi Fujita
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Phytotherapy research : PTR     Volume:  23     ISSN:  1099-1573     ISO Abbreviation:  Phytother Res     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-08-03     Completed Date:  2009-09-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8904486     Medline TA:  Phytother Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  1134-9     Citation Subset:  IM    
Copyright Information:
Copyright 2009 John Wiley & Sons, Ltd.
Affiliation:
Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, N18 W9, Kita-ku, Sapporo 060-0818, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Benzo(a)pyrene
Cacao / chemistry*
Cytochrome P-450 CYP1A1 / antagonists & inhibitors*,  metabolism
Free Radical Scavengers / pharmacology
Male
Mutagenicity Tests
Plant Extracts / pharmacology*
Rats
Rats, Wistar
Salmonella typhimurium / drug effects*
tert-Butylhydroperoxide
Chemical
Reg. No./Substance:
0/Free Radical Scavengers; 0/Plant Extracts; 50-32-8/Benzo(a)pyrene; 75-91-2/tert-Butylhydroperoxide; EC 1.14.14.1/Cytochrome P-450 CYP1A1

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