| Cruciate thinning of the zona pellucida for more successful enhancement of blastocyst hatching in the mouse. | |
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MedLine Citation:
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PMID: 1522198 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Implantation rates remain low following human in-vitro fertilization (IVF). Suboptimal culture conditions may limit the ability of embryos to hatch as blastocysts, and artificial opening of the zona pellucida has been proposed as a means to promote subsequent hatching (assisted hatching). Such techniques must have minimal adverse effects on the embryos, while maximizing the potential for an embryo to hatch fully as a blastocyst. In a mouse model, we compared embryonic development after zona drilling, and cruciate thinning of the zona (CTOZ) intended to simulate the natural thinning of the zona pellucida. Using acidic Tyrode's solution both zona drilling and cruciate-thinning were performed on day 3 morulae. On day 4 the rates of complete hatching of blastocysts were 0/165, 24/172 and 72/175 in control, zona drilled and thinned groups respectively (P less than 0.0001). On day 5 the rates of complete hatching in the same groups were 20/165, 54/172 and 120/175 respectively (P less than 0.00001) and by day 6, 66/165, 74/172 and 130/175 respectively (P less than 0.00001). The rate of arrest at the morula stage was 24/172 versus 8/175 in the zona drilled and thinned groups respectively (P less than 0.005, whilst the rate of arrest at the blastocyst stage was 21/172 versus 14/175 respectively (NS). Hence cruciate thinning of the zona appears less detrimental at the morula stage than zona drilling, but eventual rates of arrest at the blastocyst stage were comparable. Both techniques significantly increased the rate of hatching, but zona drilling did not guarantee complete hatching.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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E A Khalifa; M J Tucker; P Hunt |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Human reproduction (Oxford, England) Volume: 7 ISSN: 0268-1161 ISO Abbreviation: Hum. Reprod. Publication Date: 1992 Apr |
Date Detail:
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Created Date: 1992-10-14 Completed Date: 1992-10-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8701199 Medline TA: Hum Reprod Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 532-6 Citation Subset: IM |
Affiliation:
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Reproductive Biology Associates, Atlanta, GA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blastocyst / physiology* Embryo Implantation Female Fertilization in Vitro / methods* Isotonic Solutions / pharmacology Male Mice Micromanipulation / methods* Zona Pellucida / drug effects, physiology* |
| Chemical | |
Reg. No./Substance:
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0/Isotonic Solutions; 0/Tyrode's solution |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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