Document Detail

Crotoxin, a rattlesnake toxin, induces a long-lasting inhibitory effect on phagocytosis by neutrophils.
MedLine Citation:
PMID:  23045721     Owner:  NLM     Status:  MEDLINE    
Crotalus durissus terrificus snake venom (CdtV) has long-lasting anti-inflammatory properties and inhibits the spreading and phagocytic activity of macrophages. Crotoxin (CTX), the main component of CdtV, is responsible for these effects. Considering the role of neutrophils in the inflammatory response and the lack of information about the effect of CdtV on neutrophils, the aim of this study was to investigate the effect of CdtV and CTX on two functions of neutrophils, namely phagocytosis and production of reactive oxygen species, and on the intracellular signaling involved in phagocytosis, particularly on tyrosine phosphorylation and rearrangements of the actin cytoskeleton. Our results showed that the incubation of neutrophils with CdtV or CTX, at different concentrations, or the subcutaneous injection of CdtV or CTX in rats two hours or one, four or 14 days before or one hour after the induction of inflammation inhibited the phagocytic activity of neutrophils. Furthermore, these in vitro and in vivo effects were associated with CdtV and CTX inhibition of tyrosine phosphorylation and consequently actin polymerization. Despite the inhibitory effect on phagocytosis, this study demonstrated that CdtV and CTX did not alter the production of the main reactive oxygen species. Therefore, this study characterized, for the first time, the actions of CdtV on neutrophils and demonstrated that CTX induces a long-lasting inhibition of tyrosine phosphorylation and consequently phagocytosis. We suggest that CTX represents a potential natural product in controlling inflammatory diseases, since a single dose exerts a long-lasting effect on intracellular signaling involved in phagocytosis by neutrophils.
Tatiane S Lima; Sálua C Cataneo; Ana Caroline C Iritus; Sandra C Sampaio; Maisa S Della-Casa; Maria Cristina Cirillo
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-10-08
Journal Detail:
Title:  Experimental biology and medicine (Maywood, N.J.)     Volume:  237     ISSN:  1535-3699     ISO Abbreviation:  Exp. Biol. Med. (Maywood)     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-11-01     Completed Date:  2013-02-05     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  100973463     Medline TA:  Exp Biol Med (Maywood)     Country:  England    
Other Details:
Languages:  eng     Pagination:  1219-30     Citation Subset:  IM    
Laboratory of Pathophysiology, Butantan Institute, Av. Vital Brazil 1500, São Paulo, Brazil.
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MeSH Terms
Anti-Inflammatory Agents / pharmacology
Crotoxin / pharmacology*
Hydrogen Peroxide / metabolism
Inflammation / immunology
Leukocyte Count
Neutrophils / drug effects*,  metabolism
Phagocytosis / drug effects*
Rats, Wistar
Superoxides / metabolism
Reg. No./Substance:
0/Anti-Inflammatory Agents; 11062-77-4/Superoxides; 7722-84-1/Hydrogen Peroxide; 9007-40-3/Crotoxin

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