Document Detail

Crosstalk on cell behaviour in interactive cocultures of hMSCs with various oral cell types.
MedLine Citation:
PMID:  22881458     Owner:  NLM     Status:  Publisher    
When prospectively applied for regenerative therapies, human bone marrow-derived mesenchymal stem cells (hMSCs) interact with the locally residing host cells. With respect to the developmentally particular origin of oral cells, little is known about the putatively discriminative behavioural responses of hMSCs in interaction with various oral cell types, including human alveolar bone osteoblasts (hOAs), periodontal ligament fibroblasts (hPDLs) and gingival fibroblasts (hGFs). In order to assess the crosstalk between hMSCs and oral cells, interactive cocultures were established by combining well characterized hMSCs with hOAs, hPDLs or hGFs, and the behavioural hMSC aspects, i.e. proliferation and gene expression, were measured by employing a BrdU assay and real-time qRT-PCR, while apoptosis was quantified by in situ cell death detection kit. HMSCs expressed the typical antigen spectrum lacking CD34, CD45, CD14, CD19 and HLA-DR, while expressing CD73, CD90 and CD105, and could successfully be transformed into adipocytes, osteocytes and chondrocytes. Monocultured control hMSCs proliferated readily, whereas a general reduction of BrdU-labelled cells was observed in cocultures. Globally, upon extending time periods, interactive coculture combinations of hMSCs with hOAs reduced both osteogenic gene and stem cell marker transcription in hMSCs, a phenomenon appearing less pronounced by combining hMSCs with hPDLs, such that the observed effects in terms of proliferation and gene expression followed the same ranking: hOAs>hGFs>hPDLs. Vice versa, in interactive hMSC cocultures the cell survival rate was significantly increased, irrespective from the combined coculture cell-counterpart. Our results show for the first time that behaviour of hMSCs reflected by proliferation and gene expression was governed by interaction with various oral cells in a cell type-discriminative manner. In addition, hMSC coculture restrains apoptosis, such that influences on cell behaviour appear as a crosstalk. In summary, interactive cocultures render the basis for a prospective prediction of mutual cell behaviour in hMSC-based oral tissue regeneration disclosing that oral cells shift hMSC behaviour from proliferation to differentiation and apoptosis-repressing features.
Susanne Proksch; Thorsten Steinberg; Susanne Stampf; Ulrich Schwarz; Elmar Hellwig; Pascal Tomakidi
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-10
Journal Detail:
Title:  Tissue engineering. Part A     Volume:  -     ISSN:  1937-335X     ISO Abbreviation:  Tissue Eng Part A     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101466659     Medline TA:  Tissue Eng Part A     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
University Freiburg Medical Center, Dental School and Hospital, Dpt. of Operative Dentistry and Periodontology, Hugstetter Str. 55, Freiburg, Germany, 79106, ++49 +761 270 48430;
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