Document Detail


Crosstalk between elicitor-induced cell death and cell cycle regulation in tobacco BY-2 cells.
MedLine Citation:
PMID:  15361147     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The molecular links between cell cycle control and the regulation of programmed cell death are largely unknown in plants. Here we studied the relationship between the cell cycle and elicitor-induced cell death using synchronized tobacco BY-2 cells. Flow cytometry and fluorescence microscopy of nuclear DNA, and RNA gel-blot analyses of cell cycle-related genes revealed that the proteinaceous elicitor cryptogein induced cell cycle arrest at the G1 or G2 phase before the induction of cell death. Furthermore, the patterns of cell death induction and defence-related genes were different in different phases of the cell cycle. Constitutive treatment with cryptogein induced cell cycle arrest and cell death at the G1 or G2 phase. With transient treatment for 2 h, cell cycle arrest and cell death were only induced by treatment with the elicitor during the S or G1 phase. By contrast, the elicitor-induced production of reactive oxygen species was observed during all phases of the cell cycle. These results indicate that although recognition of the elicitor signal is cell cycle-independent, the induction of cell cycle arrest and cell death depends on the phase of the cell cycle.
Authors:
Yasuhiro Kadota; Takashi Watanabe; Shinsuke Fujii; Katsumi Higashi; Toshio Sano; Toshiyuki Nagata; Seiichiro Hasezawa; Kazuyuki Kuchitsu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Plant journal : for cell and molecular biology     Volume:  40     ISSN:  0960-7412     ISO Abbreviation:  Plant J.     Publication Date:  2004 Oct 
Date Detail:
Created Date:  2004-09-13     Completed Date:  2004-12-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9207397     Medline TA:  Plant J     Country:  England    
Other Details:
Languages:  eng     Pagination:  131-42     Citation Subset:  IM    
Affiliation:
Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan.
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MeSH Terms
Descriptor/Qualifier:
Aphidicolin / pharmacology
Cell Cycle / drug effects,  physiology*
Cell Death / drug effects,  physiology*
Cell Division / physiology
Cell Line
Plant Proteins / physiology*
Signal Transduction
Tobacco / cytology*,  genetics,  physiology*
Transcription, Genetic
Chemical
Reg. No./Substance:
0/Plant Proteins; 38966-21-1/Aphidicolin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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