| Crosstalk between Nap1 protein and Cds1 checkpoint kinase to maintain chromatin integrity. | |
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MedLine Citation:
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PMID: 18474252 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The nucleosome assembly protein Nap1 has been implicated in various cellular functions such as histone shuttling into the nucleus, nucleosome assembly, chromatin remodelling, transcriptional control and cell-cycle regulation in Saccharomyces cerevisiae. In Schizosaccharomyces pombe nap1 null mutant cells are viable but they showed a delay in the onset of mitosis which is rescued by the absence of the replication Cds1 checkpoint kinase. In contrast, the absence of the DNA-damage Chk1 checkpoint kinase is unable to rescue the delay. Moreover, the double nap1 cds1 mutant cells lose viability and cells show positive H2AX phosphorylation, suggesting that the viability of nap1-deleted cells is due to the Cds1 kinase. We also show that overexpression of Nap1 protein blocks the cell cycle in G1 phase. |
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Authors:
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Maribel Grande; Eva Lambea; Alba Fajardo; Sandra López-Avilés; Douglas Kellogg; Rosa Aligue |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-04-16 |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1783 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 2008 Sep |
Date Detail:
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Created Date: 2008-06-24 Completed Date: 2008-08-13 Revised Date: 2011-11-02 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1595-604 Citation Subset: IM |
Affiliation:
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Departament Biologia Cellular, Universitat de Barcelona, IDIBAPS. C/ Casanova 143. 08036 Barcelona, Catalunya, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Cell Cycle Proteins / genetics, metabolism* Cell Proliferation Cell Survival Chromatin / metabolism Cytoskeleton / metabolism DNA Replication G1 Phase Gene Deletion Genomic Instability Mitosis Molecular Chaperones / genetics, metabolism* Molecular Sequence Data Nuclear Proteins / genetics, metabolism* Protein-Serine-Threonine Kinases / genetics, metabolism* Schizosaccharomyces / cytology, genetics*, metabolism Schizosaccharomyces pombe Proteins / genetics, metabolism* Sequence Homology, Amino Acid |
| Chemical | |
Reg. No./Substance:
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0/Cell Cycle Proteins; 0/Chromatin; 0/Molecular Chaperones; 0/Nap1 protein, S pombe; 0/Nap2 protein, S pombe; 0/Nuclear Proteins; 0/Schizosaccharomyces pombe Proteins; EC 2.7.1.11/checkpoint kinase 2; EC 2.7.11.1/Protein-Serine-Threonine Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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