Document Detail

Crosstalk between Nap1 protein and Cds1 checkpoint kinase to maintain chromatin integrity.
MedLine Citation:
PMID:  18474252     Owner:  NLM     Status:  MEDLINE    
The nucleosome assembly protein Nap1 has been implicated in various cellular functions such as histone shuttling into the nucleus, nucleosome assembly, chromatin remodelling, transcriptional control and cell-cycle regulation in Saccharomyces cerevisiae. In Schizosaccharomyces pombe nap1 null mutant cells are viable but they showed a delay in the onset of mitosis which is rescued by the absence of the replication Cds1 checkpoint kinase. In contrast, the absence of the DNA-damage Chk1 checkpoint kinase is unable to rescue the delay. Moreover, the double nap1 cds1 mutant cells lose viability and cells show positive H2AX phosphorylation, suggesting that the viability of nap1-deleted cells is due to the Cds1 kinase. We also show that overexpression of Nap1 protein blocks the cell cycle in G1 phase.
Maribel Grande; Eva Lambea; Alba Fajardo; Sandra López-Avilés; Douglas Kellogg; Rosa Aligue
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-04-16
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1783     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-06-24     Completed Date:  2008-08-13     Revised Date:  2011-11-02    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1595-604     Citation Subset:  IM    
Departament Biologia Cellular, Universitat de Barcelona, IDIBAPS. C/ Casanova 143. 08036 Barcelona, Catalunya, Spain.
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MeSH Terms
Amino Acid Sequence
Cell Cycle Proteins / genetics,  metabolism*
Cell Proliferation
Cell Survival
Chromatin / metabolism
Cytoskeleton / metabolism
DNA Replication
G1 Phase
Gene Deletion
Genomic Instability
Molecular Chaperones / genetics,  metabolism*
Molecular Sequence Data
Nuclear Proteins / genetics,  metabolism*
Protein-Serine-Threonine Kinases / genetics,  metabolism*
Schizosaccharomyces / cytology,  genetics*,  metabolism
Schizosaccharomyces pombe Proteins / genetics,  metabolism*
Sequence Homology, Amino Acid
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Chromatin; 0/Molecular Chaperones; 0/Nap1 protein, S pombe; 0/Nap2 protein, S pombe; 0/Nuclear Proteins; 0/Schizosaccharomyces pombe Proteins; EC kinase 2; EC Kinases

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