Document Detail

Crosstalk of Escherichia coli FadR with global regulators in expression of fatty acid transport genes.
MedLine Citation:
PMID:  23029459     Owner:  NLM     Status:  MEDLINE    
Escherichia coli FadR plays two regulatory roles in fatty acid metabolism. FadR represses the fatty acid degradation (fad) system and activates the unsaturated fatty acid synthetic pathway. Cross-talk between E. coli FadR and the ArcA-ArcB oxygen-responsive two-component system was observed that resulted in diverse regulation of certain fad regulon β-oxidation genes. We have extended such analyses to the fadL and fadD genes, the protein products of which are required for long chain fatty acid transport and have also studied the role of a third global regulator, the CRP-cAMP complex. The promoters of both the fadL and fadD genes contain two experimentally validated FadR-binding sites plus binding sites for ArcA and CRP-cAMP. Despite the presence of dual binding sites FadR only modestly regulates expression of these genes, indicating that the number of binding sites does not determine regulatory strength. We report complementary in vitro and in vivo studies indicating that the CRP-cAMP complex directly activates expression of fadL and fadD as well as the β-oxidation gene, fadH. The physiological relevance of the fadL and fadD transcription data was validated by direct assays of long chain fatty acid transport.
Youjun Feng; John E Cronan
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-09-28
Journal Detail:
Title:  PloS one     Volume:  7     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2012  
Date Detail:
Created Date:  2012-10-02     Completed Date:  2013-02-21     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e46275     Citation Subset:  IM    
Department of Microbiology, University of Illinois, Urbana, Illinois, United States of America.
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MeSH Terms
Bacterial Outer Membrane Proteins / genetics*,  metabolism
Bacterial Proteins / genetics*,  metabolism
Binding Sites
Biological Transport
Coenzyme A Ligases / genetics*,  metabolism
Cyclic AMP / metabolism*
Cyclic AMP Receptor Protein / genetics*,  metabolism
Escherichia coli / genetics*,  metabolism
Escherichia coli Proteins / genetics*,  metabolism
Fatty Acid Transport Proteins / genetics*,  metabolism
Fatty Acids / metabolism*
Gene Expression Regulation, Bacterial*
Membrane Proteins / genetics,  metabolism
Oxygen / metabolism
Promoter Regions, Genetic
Protein Binding
Protein Kinases / genetics,  metabolism
Repressor Proteins / genetics*,  metabolism
Signal Transduction
Transcription, Genetic
Grant Support
Reg. No./Substance:
0/Bacterial Outer Membrane Proteins; 0/Bacterial Proteins; 0/Cyclic AMP Receptor Protein; 0/Escherichia coli Proteins; 0/FadR protein, Bacteria; 0/Fatty Acid Transport Proteins; 0/Fatty Acids; 0/Membrane Proteins; 0/Repressor Proteins; 0/arcA protein, E coli; 0/crp protein, E coli; 0/fadL protein, E coli; 60-92-4/Cyclic AMP; 7782-44-7/Oxygen; EC 2.7.-/Protein Kinases; EC 2.7.3.-/arcB protein, E coli; EC 6.2.1.-/Coenzyme A Ligases; EC 6.2.1.-/acyl-coenzyme A synthetase, E coli

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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