| Crosstalk of Escherichia coli FadR with global regulators in expression of fatty acid transport genes. | |
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MedLine Citation:
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PMID: 23029459 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Escherichia coli FadR plays two regulatory roles in fatty acid metabolism. FadR represses the fatty acid degradation (fad) system and activates the unsaturated fatty acid synthetic pathway. Cross-talk between E. coli FadR and the ArcA-ArcB oxygen-responsive two-component system was observed that resulted in diverse regulation of certain fad regulon β-oxidation genes. We have extended such analyses to the fadL and fadD genes, the protein products of which are required for long chain fatty acid transport and have also studied the role of a third global regulator, the CRP-cAMP complex. The promoters of both the fadL and fadD genes contain two experimentally validated FadR-binding sites plus binding sites for ArcA and CRP-cAMP. Despite the presence of dual binding sites FadR only modestly regulates expression of these genes, indicating that the number of binding sites does not determine regulatory strength. We report complementary in vitro and in vivo studies indicating that the CRP-cAMP complex directly activates expression of fadL and fadD as well as the β-oxidation gene, fadH. The physiological relevance of the fadL and fadD transcription data was validated by direct assays of long chain fatty acid transport. |
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Authors:
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Youjun Feng; John E Cronan |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2012-09-28 |
Journal Detail:
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Title: PloS one Volume: 7 ISSN: 1932-6203 ISO Abbreviation: PLoS ONE Publication Date: 2012 |
Date Detail:
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Created Date: 2012-10-02 Completed Date: 2013-02-21 Revised Date: 2013-03-01 |
Medline Journal Info:
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Nlm Unique ID: 101285081 Medline TA: PLoS One Country: United States |
Other Details:
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Languages: eng Pagination: e46275 Citation Subset: IM |
Affiliation:
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Department of Microbiology, University of Illinois, Urbana, Illinois, United States of America. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Outer Membrane Proteins
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genetics*,
metabolism Bacterial Proteins / genetics*, metabolism Binding Sites Biological Transport Coenzyme A Ligases / genetics*, metabolism Cyclic AMP / metabolism* Cyclic AMP Receptor Protein / genetics*, metabolism Escherichia coli / genetics*, metabolism Escherichia coli Proteins / genetics*, metabolism Fatty Acid Transport Proteins / genetics*, metabolism Fatty Acids / metabolism* Gene Expression Regulation, Bacterial* Membrane Proteins / genetics, metabolism Oxidation-Reduction Oxygen / metabolism Promoter Regions, Genetic Protein Binding Protein Kinases / genetics, metabolism Regulon Repressor Proteins / genetics*, metabolism Signal Transduction Transcription, Genetic |
| Grant Support | |
ID/Acronym/Agency:
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AI15650/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Outer Membrane Proteins; 0/Bacterial Proteins; 0/Cyclic AMP Receptor Protein; 0/Escherichia coli Proteins; 0/FadR protein, Bacteria; 0/Fatty Acid Transport Proteins; 0/Fatty Acids; 0/Membrane Proteins; 0/Repressor Proteins; 0/arcA protein, E coli; 0/crp protein, E coli; 0/fadL protein, E coli; 60-92-4/Cyclic AMP; 7782-44-7/Oxygen; EC 2.7.-/Protein Kinases; EC 2.7.3.-/arcB protein, E coli; EC 6.2.1.-/Coenzyme A Ligases; EC 6.2.1.-/acyl-coenzyme A synthetase, E coli |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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