Document Detail


Crosslinking of phenylboronic acid receptors as a means of glucose selective holographic detection.
MedLine Citation:
PMID:  16414255     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A holographic sensor for the detection of glucose has been developed that is based on a hydrogel film containing phenylboronic acid receptors. Changes to the replay wavelength of the hologram were used to characterise the swelling and de-swelling behaviour of the hydrogel matrix upon receptor-ligand binding. The effect of introducing a fixed positive charge into the polymer matrix by modification of the hydrogel with a quaternary amine group (3-acrylamidopropyl)trimethylammonium chloride (ATMA), was investigated for a range of sugars and the alpha-hydroxy acid, lactate, at physiological pH. The quaternary amine-modified hydrogel matrix was found to contract in the presence of glucose, whereas, it was minimally responsive to other saccharides. The selectivity of the sensor for glucose compared to lactate was also significantly improved compared to the unmodified film. A crosslinking mechanism is proposed to explain the enhanced selectivity to glucose.
Authors:
Adrian M Horgan; Alexander J Marshall; Simon J Kew; Kathryn E S Dean; Chris D Creasey; Satyamoorthy Kabilan
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Publication Detail:
Type:  Evaluation Studies; Journal Article     Date:  2006-01-18
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  21     ISSN:  0956-5663     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-02-21     Completed Date:  2006-06-08     Revised Date:  2009-07-14    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  England    
Other Details:
Languages:  eng     Pagination:  1838-45     Citation Subset:  IM    
Affiliation:
Smart Holograms Ltd., 291 Cambridge Science Park, Milton Road, Cambridge, CB4 0WF, UK. adrian.horgan@smartholograms.com
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MeSH Terms
Descriptor/Qualifier:
Biosensing Techniques / methods*
Boronic Acids / analysis,  chemistry*
Cross-Linking Reagents
Glucose / analysis*,  chemistry
Holography / methods*
Refractometry / methods*
Reproducibility of Results
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Boronic Acids; 0/Cross-Linking Reagents; 50-99-7/Glucose; 98-80-6/benzeneboronic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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