| Cross-talk between cAMP and p38 MAPK pathways in the induction of leptin by hCG in human placental syncytiotrophoblasts. | |
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MedLine Citation:
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PMID: 21562093 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Leptin produced by the placental syncytiotrophoblasts participates in a number of processes in pregnancy including implantation, proliferation of the cytotrophoblasts as well as nutrient transfer across the placenta. Despite the functional significance of leptin in pregnancy, the regulation of leptin synthesis is poorly understood in human placental syncytiotrophoblasts. In this study we investigated the role of endogenous hCG in the regulation of leptin production as well as the underlying mechanism involving the crosstalk between cAMP and p38 MAPK pathways. We found that neutralization of endogenous hCG with its antibody dose-dependently decreased leptin mRNA level and secretion, while exogenous hCG increased leptin mRNA level and secretion. Activation of the cAMP pathway with dibutyryl cAMP (db cAMP) or forskolin recapitulated the stimulatory effect of hCG on leptin expression. Inhibition of protein kinase A (PKA) with H89 not only reduced the basal leptin expression, but also attenuated the leptin-induced expression by hCG. Treatment of the syncytiotrophoblasts with db cAMP and hCG phosphorylated p38 MAPK. Inhibition of p38 MAPK with SB203580 not only reduced the basal leptin production, but also attenuated the leptin-induced production by both hCG and db cAMP. These data suggest that endogenous hCG plays a significant role in maintaining leptin production in human placental syncytiotrophoblasts, and this effect involves a crosstalk between cAMP and p38 MAPK pathways. |
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Authors:
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Yuchun Ge; Jianneng Li; Xiaotian Ni; Chunming Guo; Wangsheng Wang; Tao Duan; Kang Sun |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-5-11 |
Journal Detail:
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Title: Reproduction (Cambridge, England) Volume: - ISSN: 1741-7899 ISO Abbreviation: - Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-5-12 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100966036 Medline TA: Reproduction Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Y Ge, Shanghai First Maternity and Infant Health Hospital, Shanghai, China. |
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