Document Detail


Cross-species analysis of nicotine-induced proteomic alterations in pancreatic cells.
MedLine Citation:
PMID:  23456891     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Toxic compounds in tobacco, such as nicotine, may adversely affect pancreatic function. We aim to determine nicotine-induced protein alterations in pancreatic cells, thereby revealing links between nicotine exposure and pancreatic disease. We compared the proteomic alterations induced by nicotine treatment in cultured pancreatic cells (mouse, rat, and human stellate cells and human duct cells) using MS-based techniques, specifically SDS-PAGE (gel) coupled with LC-MS/MS and spectral counting. We identified thousands of proteins in pancreatic cells, hundreds of which were identified exclusively or in higher abundance in either nicotine-treated or untreated cells. Interspecies comparisons of stellate cell proteins revealed several differentially abundant proteins (in nicotine treated versus untreated cells) common among the three species. Proteins appearing in all nicotine-treated stellate cells include amyloid beta (A4), procollagen type VI alpha 1, integral membrane protein 2B, and toll-interacting protein. Proteins that were differentially expressed upon nicotine treatment across cell lines were enriched in certain pathways, including nicotinic acetylcholine receptor, cytokine, and integrin signaling. At this analytical depth, we conclude that similar pathways are affected by nicotine, but alterations at the protein level among stellate cells of different species vary. Further interrogation of such pathways will lead to insights into the potential effect of nicotine on pancreatic cells at the biomolecular level and the extension of this concept to the effect of nicotine on pancreatic disease.
Authors:
Joao A Paulo; Raul Urrutia; Vivek Kadiyala; Peter Banks; Darwin L Conwell; Hanno Steen
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Proteomics     Volume:  13     ISSN:  1615-9861     ISO Abbreviation:  Proteomics     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-04-22     Completed Date:  2013-12-10     Revised Date:  2014-05-29    
Medline Journal Info:
Nlm Unique ID:  101092707     Medline TA:  Proteomics     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1499-512     Citation Subset:  IM    
Copyright Information:
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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MeSH Terms
Descriptor/Qualifier:
Amyloid beta-Peptides / metabolism
Animals
Cell Line
Cells, Cultured
Collagen Type VI / metabolism
Electrophoresis, Polyacrylamide Gel
Humans
Membrane Glycoproteins / metabolism
Mice
Nicotine / pharmacology*
Pancreas / cytology*,  drug effects*,  metabolism
Pancreatic Stellate Cells / drug effects,  metabolism
Proteins / analysis,  metabolism*
Rats
Signal Transduction / drug effects
Species Specificity
Tandem Mass Spectrometry / methods
Grant Support
ID/Acronym/Agency:
1 F32 DK085835-01A1/DK/NIDDK NIH HHS; 1 R21 DK081703-01A2/DK/NIDDK NIH HHS; 5 P30 DK034854-24/DK/NIDDK NIH HHS; F32 DK085835/DK/NIDDK NIH HHS; P30 DK034854/DK/NIDDK NIH HHS; R01 DK052913/DK/NIDDK NIH HHS; R21 DK081703/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Amyloid beta-Peptides; 0/Collagen Type VI; 0/ITM2B protein, human; 0/Membrane Glycoproteins; 0/Proteins; 54-11-5/Nicotine
Comments/Corrections
Comment In:
Proteomics. 2013 May;13(9):1379-80   [PMID:  23606677 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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