Document Detail

Cross-reactive carbohydrate determinants strongly affect the results of the basophil activation test in hymenoptera-venom allergy.
MedLine Citation:
PMID:  20545702     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: In hymenoptera-venom allergy, sera of up to 60% of patients show in vitro reactivity to honeybee venom (HBV) and yellow jacket venom (YJV). This phenomenon is mainly caused by specific IgE (sIgE) against cross-reactive carbohydrate determinants (CCD). Whether or not these antibodies can induce clinical symptoms is a longstanding debate.
OBJECTIVE: The aim of this study was to investigate the biological activity of CCD-sIgE and the suitability of the basophil activation test (BAT) in hymenoptera venom-allergic patients having CCD-sIgE.
METHODS: The biological activity of CCD-sIgE was analysed by application of native and CCD-depleted YJV and HBV in BAT with the blood of 62 hymenoptera venom-allergic patients and 16 non-allergic controls. According to results of intracutaneous skin tests (IC) with YJV and HBV and the existence of CCD-sIgE, patients were classified into six subgroups.
RESULTS: In patients with mono-positive IC and CCD-sIgE, and thus double-positive sIgE, BAT with native venoms was also double positive in up to 67% of the patients. In contrast, BAT with CCD-depleted venoms was positive only with the IC-positive venom. However, activation of basophils with the IC-negative venom was significantly lower compared with the IC-positive one. In IC mono-positive patients without CCD-sIgE, BAT was mono-positive with the IC-positive venom in the native and in the CCD-depleted form. CCD-positive patients with double-positive IC were a heterogeneous group, with the majority of CCD-positive patients also being double positive with the native forms of both venoms but mono-positive with the CCD-depleted ones.
CONCLUSIONS: In vitro BAT clearly demonstrates biological activity of CCD-sIgE. However, because most of the patients showed a mono-positive IC and activation of basophils with the IC-negative venom was significantly lower compared with the IC-positive one, the present data suggest that CCD-sIgE is clinically irrelevant in these patients.
M Mertens; S Amler; B M Moerschbacher; R Brehler
Publication Detail:
Type:  Journal Article     Date:  2010-06-07
Journal Detail:
Title:  Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology     Volume:  40     ISSN:  1365-2222     ISO Abbreviation:  Clin. Exp. Allergy     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-12     Completed Date:  2011-01-25     Revised Date:  2011-05-31    
Medline Journal Info:
Nlm Unique ID:  8906443     Medline TA:  Clin Exp Allergy     Country:  England    
Other Details:
Languages:  eng     Pagination:  1333-45     Citation Subset:  IM    
Department of Dermatology, University of Münster, Von-Esmarch-Strasse 58, Münster, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Allergens / immunology*
Antibody Specificity
Basophils / immunology*
Bee Venoms / immunology*
Carbohydrates / immunology*
Cross Reactions
Diagnosis, Differential
Hypersensitivity / diagnosis*
Immunoglobulin E / immunology
Immunologic Tests / methods*
Middle Aged
Sensitivity and Specificity
Wasp Venoms / immunology*
Reg. No./Substance:
0/Allergens; 0/Bee Venoms; 0/Carbohydrates; 0/Wasp Venoms; 37341-29-0/Immunoglobulin E
Comment In:
Clin Exp Allergy. 2010 Sep;40(9):1290-2   [PMID:  20701613 ]
Clin Exp Allergy. 2011 May;41(5):759-61   [PMID:  21457167 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Human monocytic cells direct the robust release of CXCL10 by bronchial epithelial cells during rhino...
Next Document:  Impact of a novel nutritional formula on asthma control and biomarkers of allergic airway inflammati...