| Cross-reactive carbohydrate determinants strongly affect the results of the basophil activation test in hymenoptera-venom allergy. | |
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MedLine Citation:
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PMID: 20545702 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: In hymenoptera-venom allergy, sera of up to 60% of patients show in vitro reactivity to honeybee venom (HBV) and yellow jacket venom (YJV). This phenomenon is mainly caused by specific IgE (sIgE) against cross-reactive carbohydrate determinants (CCD). Whether or not these antibodies can induce clinical symptoms is a longstanding debate. OBJECTIVE: The aim of this study was to investigate the biological activity of CCD-sIgE and the suitability of the basophil activation test (BAT) in hymenoptera venom-allergic patients having CCD-sIgE. METHODS: The biological activity of CCD-sIgE was analysed by application of native and CCD-depleted YJV and HBV in BAT with the blood of 62 hymenoptera venom-allergic patients and 16 non-allergic controls. According to results of intracutaneous skin tests (IC) with YJV and HBV and the existence of CCD-sIgE, patients were classified into six subgroups. RESULTS: In patients with mono-positive IC and CCD-sIgE, and thus double-positive sIgE, BAT with native venoms was also double positive in up to 67% of the patients. In contrast, BAT with CCD-depleted venoms was positive only with the IC-positive venom. However, activation of basophils with the IC-negative venom was significantly lower compared with the IC-positive one. In IC mono-positive patients without CCD-sIgE, BAT was mono-positive with the IC-positive venom in the native and in the CCD-depleted form. CCD-positive patients with double-positive IC were a heterogeneous group, with the majority of CCD-positive patients also being double positive with the native forms of both venoms but mono-positive with the CCD-depleted ones. CONCLUSIONS: In vitro BAT clearly demonstrates biological activity of CCD-sIgE. However, because most of the patients showed a mono-positive IC and activation of basophils with the IC-negative venom was significantly lower compared with the IC-positive one, the present data suggest that CCD-sIgE is clinically irrelevant in these patients. |
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Authors:
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M Mertens; S Amler; B M Moerschbacher; R Brehler |
Publication Detail:
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Type: Journal Article Date: 2010-06-07 |
Journal Detail:
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Title: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology Volume: 40 ISSN: 1365-2222 ISO Abbreviation: Clin. Exp. Allergy Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-08-12 Completed Date: 2011-01-25 Revised Date: 2011-05-31 |
Medline Journal Info:
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Nlm Unique ID: 8906443 Medline TA: Clin Exp Allergy Country: England |
Other Details:
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Languages: eng Pagination: 1333-45 Citation Subset: IM |
Affiliation:
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Department of Dermatology, University of Münster, Von-Esmarch-Strasse 58, Münster, Germany. melanie.mertens@ukmuenster.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Aged Allergens / immunology* Antibody Specificity Basophils / immunology* Bee Venoms / immunology* Carbohydrates / immunology* Cross Reactions Diagnosis, Differential Female Humans Hypersensitivity / diagnosis* Immunoglobulin E / immunology Immunologic Tests / methods* Male Middle Aged Sensitivity and Specificity Wasp Venoms / immunology* |
| Chemical | |
Reg. No./Substance:
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0/Allergens; 0/Bee Venoms; 0/Carbohydrates; 0/Wasp Venoms; 37341-29-0/Immunoglobulin E |
| Comments/Corrections | |
Comment In:
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Clin Exp Allergy. 2010 Sep;40(9):1290-2
[PMID:
20701613
]
Clin Exp Allergy. 2011 May;41(5):759-61 [PMID: 21457167 ] |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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