Document Detail


Cross-links in cell walls of Bacillus subtilis by rotational-echo double-resonance 15N NMR.
MedLine Citation:
PMID:  8103049     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cross-link index of peptidoglycan of intact cell walls of Bacillus subtilis grown in media containing L-[2-13C,15N]aspartic acid has been determined by rotational-echo double-resonance 15N NMR. A cross-link index of 72% decreased to 47% when the bacteria were exposed to the antibiotic cephalosporin C. The fraction of 15N label routed from aspartic acid in the media to glutamic acid in the peptidoglycan peptide stems more than doubled for the cephalosporin-exposed cells, indicating interference with general cell wall nitrogen metabolism by the antibiotic.
Authors:
Y Pan; N S Shenouda; G E Wilson; J Schaefer
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  268     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1993 Sep 
Date Detail:
Created Date:  1993-09-30     Completed Date:  1993-09-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  18692-5     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Washington University, St. Louis, Missouri 63130.
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MeSH Terms
Descriptor/Qualifier:
Aspartic Acid / metabolism
Bacillus subtilis / chemistry*,  drug effects,  metabolism
Cell Wall / chemistry*,  metabolism
Cephalosporins / pharmacology
Glutamates / metabolism
Glutamic Acid
Magnetic Resonance Spectroscopy*
Nitrogen / metabolism
Nitrogen Isotopes
Peptidoglycan / chemistry*
Chemical
Reg. No./Substance:
0/Cephalosporins; 0/Glutamates; 0/Nitrogen Isotopes; 0/Peptidoglycan; 56-84-8/Aspartic Acid; 56-86-0/Glutamic Acid; 61-24-5/cephalosporin C; 7727-37-9/Nitrogen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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