| Crocin Triggers the Apoptosis Through Increasing the Bax/Bcl-2 Ratio and Caspase Activation in Human Gastric Adenocarcinoma, AGS, Cells. | |
| | |
MedLine Citation:
|
PMID: 23347444 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
We previously showed the anticancer property of crocin, a carotenoid isolated and purified from saffron against chemical-induced gastric and breast cancer in rats. In this study, the mechanism of crocin action was investigated in the gastric adenocarcinoma (AGS) cells in comparison with human normal fibroblast skin cells (HFSF-PI3). Crocin revealed a dose- and time-dependent cytotoxic effect against an AGS cell line, as determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Crocin-induced apoptosis was evidenced by flow cytometry and measuring caspase activity. The increased sub-G1 population and activated caspases in the treated AGS cells confirmed its anticancer effect. Expression of both Bax and Bcl-2 was determined using a semiquantitative reverse transcriptase-polymerase chain reaction and Western blot in these cells before and after treatment with crocin. Apoptosis was significantly stimulated as indicated by increasing the Bax/Bcl-2 ratio after crocin treatment. All of the above-mentioned parameters remained normal in HFSF-PI3 treated with crocin. These data are providing insight into the molecular mechanisms underlying the crocin-induced apoptosis in the AGS cells, rendering it as the potential anticancer agent. |
| | |
Authors:
|
Reyhane Hoshyar; S Zahra Bathaie; Majid Sadeghizadeh |
Related Documents
:
|
20979154 - Assessment of phosphamidon-induced apoptosis in human peripheral blood mononuclear cell... 23340174 - Anti-apoptotic proteins on guard of melanoma cell survival. 23280844 - Genetic deletion of cd36 enhances injury after acute neonatal stroke. 21191074 - Il-1α modulates neutrophil recruitment in chronic inflammation induced by hydrocarbon ... 23606514 - Body mass index is not associated with cytokine induction during experimental human end... 20214504 - Anti-ischemic and anti-inflammatory activity of (s)-cis-verbenol. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: DNA and cell biology Volume: 32 ISSN: 1557-7430 ISO Abbreviation: DNA Cell Biol. Publication Date: 2013 Feb |
Date Detail:
|
Created Date: 2013-01-25 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9004522 Medline TA: DNA Cell Biol Country: United States |
Other Details:
|
Languages: eng Pagination: 50-7 Citation Subset: IM |
Affiliation:
|
1 Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University , Tehran, Iran . |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Signaling molecules involved in lipid-induced pancreatic beta-cell dysfunction.
Next Document: Effects of the Adverse Life Events and Disrupted in Schizophrenia-1 (DISC1) Gene Polymorphisms on Ac...