Document Detail


Crocidolite induces cell transformation and p53 gene mutation in BALB/c-3T3 cells.
MedLine Citation:
PMID:  10992274     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell transformation is one of the most common assays used to study morphological changes in the multistep process of carcinogenesis. The present study was initiated to investigate the ability of crocidolite to induce cell transformation in BALB/c-3T3 cells and to analyze the relationship between p53 mutations and crocidolite-induced cell transformation, if any. Cell transformation was carried out according to standard procedures. Exponentially growing cells were exposed to different concentrations (0.2-20 microg/cm(2)) of crocidolite fibers for 72 h. Foci obtained from cell transformation were analyzed for their ability to grow in soft agar (anchorage-independence) and p53 alterations. The results of this study demonstrate that there was an increase in transformation frequency (TF) with an increase in concentration of crocidolite. Also, focal cells were able to grow on soft agar, indicating anchorage-independence. cDNA was prepared from RNA isolated from Type 3 foci and subjected to mutational analysis. Eleven exons of the p53 gene from eight transformed cell lines were analyzed for alterations using polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP). Alterations were found in seven of eight cell lines, two of them were in exons 4-6, and five in exons 9-11. The alterations were randomly scattered among the crocidolite dose groups. These results suggest that crocidolite induces mutations predominantly in exons 9-11 of the p53 gene in a nondose-dependent manner.
Authors:
F Lin; Y Liu; Y Liu; N Keshava; S Li
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Teratogenesis, carcinogenesis, and mutagenesis     Volume:  20     ISSN:  0270-3211     ISO Abbreviation:  Teratog., Carcinog. Mutagen.     Publication Date:  2000  
Date Detail:
Created Date:  2000-10-17     Completed Date:  2000-10-30     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8100917     Medline TA:  Teratog Carcinog Mutagen     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  273-81     Citation Subset:  IM    
Affiliation:
School of Public Health, West China University of Medical Science, Chengdu, Sichuan, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Animals
Asbestos, Crocidolite / toxicity*
Cell Adhesion
Cell Survival / drug effects
Cell Transformation, Neoplastic / drug effects*
Genes, p53 / drug effects*
Mice
Mice, Inbred BALB C
Mutagenesis*
Mutagenicity Tests
Polymerase Chain Reaction
Polymorphism, Single-Stranded Conformational
Reverse Transcriptase Polymerase Chain Reaction
Chemical
Reg. No./Substance:
12001-28-4/Asbestos, Crocidolite

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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