| Critical role of poly(ADP-ribose) polymerase-1 in modulating the mode of cell death caused by continuous oxidative stress. | |
| | |
MedLine Citation:
|
PMID: 19711368 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Continuously generated hydrogen peroxide (H(2)O(2)) inhibits typical apoptosis and instead initiates a caspase-independent, apoptosis-inducing factor (AIF)-mediated pyknotic cell death. This may be related to H(2)O(2)-mediated DNA damage and subsequent ATP depletion, although the exact mechanisms by which the mode of cell death is decided after H(2)O(2) exposure are still unclear. Accumulated evidence and our previous data led us to hypothesize that continuously generated H(2)O(2), not an H(2)O(2) bolus, induces severe DNA damage, signaling poly(ADP-ribose) polymerase-1 (PARP-1) activation, ATP depletion, and eventually caspase-independent cell death. Results from the present study support that H(2)O(2) generated continuously by glucose oxidase causes excessive DNA damage and PARP-1 activation. Blockage of PARP-1 by a siRNA transfection or by pharmacological inhibitor resulted in the significant inhibition of ATP depletion, loss of mitochondrial membrane potential, nuclear translocation of AIF and endonuclease G, and eventually conversion to caspase-dependent apoptosis. Overall, the current study demonstrates the different roles of PARP-1 inhibition in modulation of cell death according to the method of H(2)O(2) exposure, that is, continuous generation versus a direct addition. |
| | |
Authors:
|
Young-Ok Son; Sung-Ho Kook; Yong-Suk Jang; Xianglin Shi; Jeong-Chae Lee |
Related Documents
:
|
10963298 - Apoptosis of ng108-15 cells induced by buprenorphine hydrochloride occurs via the caspa... 9826028 - Triptolide induces apoptotic death of t lymphocyte. 14555228 - Anti-apoptotic effect of hiv protease inhibitors via direct inhibition of calpain. 16026508 - Cardiotoxin iii induces apoptosis in k562 cells through a mitochondrial-mediated pathway. 22497888 - The role of microglia in the neurogenesis of zebrafish retina. 21624938 - Hemophagocytosis causes a consumptive anemia of inflammation. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of cellular biochemistry Volume: 108 ISSN: 1097-4644 ISO Abbreviation: J. Cell. Biochem. Publication Date: 2009 Nov |
Date Detail:
|
Created Date: 2009-10-26 Completed Date: 2010-02-17 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8205768 Medline TA: J Cell Biochem Country: United States |
Other Details:
|
Languages: eng Pagination: 989-97 Citation Subset: IM |
Copyright Information:
|
(c) 2009 Wiley-Liss, Inc. |
Affiliation:
|
Graduate Center for Toxicology, University of Kentucky, Lexington, Kentucky 40536-0001, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adenosine Triphosphate
/
chemistry Apoptosis Caspases / metabolism Cell Death Cell Line, Tumor Comet Assay DNA Damage Endodeoxyribonucleases / metabolism Gene Expression Regulation* Glucose Oxidase / metabolism Humans Hydrogen Peroxide / chemistry Mitochondria / metabolism Oxidative Stress* Poly(ADP-ribose) Polymerases / metabolism* |
| Chemical | |
Reg. No./Substance:
|
56-65-5/Adenosine Triphosphate; 7722-84-1/Hydrogen Peroxide; EC 1.1.3.4/Glucose Oxidase; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.1.-/Endodeoxyribonucleases; EC 3.1.21.-/endonuclease G; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: MUC1 is a substrate for gamma-secretase.
Next Document: Regenerative medicine in the treatment of peripheral arterial disease.