| Critical role for free radicals on sprint exercise-induced CaMKII and AMPKα phosphorylation in human skeletal muscle. | |
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MedLine Citation:
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PMID: 23288553 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The extremely high energy demand elicited by sprint exercise is satisfied by an increase in oxygen consumption combined with a high glycolytic rate, leading to a marked lactate accumulation, increased AMP/ATP ratio, reduced NAD(+)/NADH.H(+) and muscle pH, which are accompanied by marked Thr(172)-AMPKα phosphorylation during the recovery period. To determine the role played by Reactive nitrogen and oxygen species (RNOS) on Thr(172)-AMPKα phosphorylation in response to cycling sprint exercise, nine voluntaries performed a single 30s sprint (Wingate test) in two occasions: one two hours after the ingestion of placebo (P) and another following the intake of antioxidants (A) (α-lipoic acid, vitamin C, and vitamin E), with a double blind design. Vastus lateralis muscle biopsies were obtained before, immediately after, 30 and 120 min post-sprint. Performance, muscle metabolism was similar during both sprints. The NAD(+)/NADH.H(+) ratio was similarly reduced (84%), and the AMP/ATP ratio similarly increased (x21 fold) immediately after the sprints. Thr(286)-CaMKII and Thr(172)-AMPKα phosphorylations were increased after the control sprint (P), but not when the sprints were preceded by the ingestion of antioxidants. Ser(485)-AMPKα1/Ser(491)-AMPKα2 phosphorylation, a known inhibitory mechanism of Thr(172)-AMPKα phosphorylation, was increased only in A. In conclusion, RNOS play a crucial role in AMPK-mediated signaling after sprint exercise in human skeletal muscle. Antioxidant ingestion two hours prior to sprint exercise abrogates the Thr(172)-AMPKα phosphorylation response observed after the ingestion of placebo by reducing CaMKII and increasing Ser(485)-AMPKα1/Ser(491)-AMPKα2 phosphorylation. Sprint performance, muscle metabolism, and the AMP/ATP and NAD(+)/NADH.H(+) ratios are not affected by the acute ingestion of antioxidants. |
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Authors:
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David Morales-Alamo; Jesus Gustavo Ponce-Gonzalez; Amelia Guadalupe-Grau; Lorena Rodriguez-Garcia; Alfredo Santana; Roser Cussó; Mario Guerrero; Cecilia Dorado; Borja Guerra; Jose A L Calbet |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2013-1-3 |
Journal Detail:
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Title: Journal of applied physiology (Bethesda, Md. : 1985) Volume: - ISSN: 1522-1601 ISO Abbreviation: J. Appl. Physiol. Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2013-1-4 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8502536 Medline TA: J Appl Physiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1University of Las Palmas de Gran Canaria. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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