Critical role of bicarbonate in calcium release from bone.

Critical role of bicarbonate in calcium release from bone.

MedLine Citation:	PMID: 1415579 Owner: NLM Status: MEDLINE
Abstract/OtherAbstract:	Calcium release from cultured bone is pH dependent; net calcium flux (JCa) from bone increases with decreasing pH. At a similar decrement in pH there is greater JCa when acidosis is produced by a low medium bicarbonate concentration ([HCO3-]), a model of metabolic acidosis (Met), compared with an increased medium PCO2, a model of respiratory acidosis (Resp). To separate the role of [HCO3-] from that of pH in inducing JCa we cultured calvariae for 3 h under three different neutral (pH approximately 7.4) isohydric environments [control (Ctl), fully compensated Met (C-Met), or fully compensated Resp (C-Resp)] and two different acid (pH approximately 7.1) isohydric environments (Met or Resp). During neutral pH (Ctl, C-Met, and C-Resp) there was JCa from bone during C-Met (decreased [HCO3-]), no net flux during Ctl (normal [HCO3-]), and JCa into bone during C-Resp (increased [HCO3-]); and JCa was correlated inversely with [HCO3-] (r = -0.824, n = 36, P less than 0.001). During acid pH there was greater JCa from bone during Met (decreased [HCO3-]) than during Resp (normal [HCO3-]); and JCa was again correlated inversely with [HCO3-] (r = -0.848, n = 22, P less than 0.001). JCa from bone during Met and Resp was greater than C-Met and C-Resp, respectively. The addition of the osteoclastic inhibitor salmon calcitonin did not alter the relative JCa results. Thus at a constant pH the magnitude of JCa from cultured neonatal mouse calvariae appears dependent on the [HCO3-]; the lower the [HCO3-], the greater the calcium efflux.(ABSTRACT TRUNCATED AT 250 WORDS)

Authors:	D A Bushinsky; N E Sessler
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Publication Detail:	Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.
Journal Detail:	Title: The American journal of physiology Volume: 263 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1992 Sep
Date Detail:	Created Date: 1992-10-29 Completed Date: 1992-10-29 Revised Date: 2007-11-14
Medline Journal Info:	Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES
Other Details:	Languages: eng Pagination: F510-5 Citation Subset: IM
Affiliation:	Nephrology Unit, University of Rochester, School of Medicine and Dentistry, New York 14642.
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MeSH Terms
Descriptor/Qualifier:	Acidosis / metabolism, pathology Acidosis, Respiratory / metabolism, pathology Animals Bicarbonates / metabolism* Bone and Bones / cytology, metabolism, pathology Calcium / metabolism Hydrogen-Ion Concentration Mice Mice, Inbred Strains Osteoclasts / physiology
Grant Support
ID/Acronym/Agency:	AM-33949/AM/NIADDK NIH HHS; AR-39906/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:	0/Bicarbonates; 7440-70-2/Calcium

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